PMID- 2161908
OWN - NLM
STAT- MEDLINE
DCOM- 19900716
LR  - 20131121
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 21
IP  - 3
DP  - 1990 Apr
TI  - A role for cAMP in the development of functional neuromuscular transmission.
PG  - 414-26
AB  - We have found that the incidence of functionally connected neuron-myotube pairs 
      in chick ciliary-myotube cultures increases from 58% to more than 90% when the 
      cells are treated for several hours with 8-bromo-cyclic adenosine monophosphate 
      (8-br-cAMP) or with agents known to increase intracellular cAMP. The increase in 
      connectivity was not accompanied by a change in neuron survival, or in the length 
      of neurite-myotube contact. Moreover, there was no change in the shape of the 
      presynaptic action potential, in mean end plate potential (epp) amplitude or in 
      the sensitivity of postsynaptic acetylcholine receptors (AChRs). One 
      interpretation of these results in that a cAMP-dependent phosphorylation acts as 
      a trigger to activate a previously "silent" synapse.
FAU - Dubinsky, J M
AU  - Dubinsky JM
AD  - Department of Anatomy and Neurobiology, Washington University School of Medicine, 
      St. Louis, Missouri 63110.
FAU - Fischbach, G D
AU  - Fischbach GD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Receptors, Cholinergic)
RN  - 1F7A44V6OU (Colforsin)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - Acetylcholine/pharmacology
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chickens
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/*physiology
MH  - Ganglia, Parasympathetic/cytology/drug effects/*physiology
MH  - Motor Neurons/cytology/drug effects/*physiology
MH  - Muscles/cytology/drug effects/*physiology
MH  - Neuromuscular Junction/drug effects/*physiology
MH  - Receptors, Cholinergic/metabolism
MH  - Synaptic Transmission
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
AID - 10.1002/neu.480210304 [doi]
PST - ppublish
SO  - J Neurobiol. 1990 Apr;21(3):414-26. doi: 10.1002/neu.480210304.