PMID- 21619712
OWN - NLM
STAT- MEDLINE
DCOM- 20110913
LR  - 20211020
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 8
DP  - 2011 May 30
TI  - Identification of CD8+ cytotoxic T lymphocyte epitopes from porcine reproductive 
      and respiratory syndrome virus matrix protein in BALB/c mice.
PG  - 263
LID - 10.1186/1743-422X-8-263 [doi]
AB  - Twenty-seven nanopeptides derived from the matrix (M) protein of porcine 
      reproductive and respiratory syndrome virus (PRRSV) were screened for their 
      ability to elicit a recall interferon-gamma (IFN-gamma) response from the splenocytes of 
      BALB/c mice following DNA vaccination and a booster vaccination with recombinant 
      vaccinia virus rWR-PRRSV-M. We identified two peptides (amino acid residues 
      K93FITSRCRL and F57GYMTFVHF) as CD8+ cytotoxic T lymphocyte (CTL) epitopes. These 
      peptides elicited significant numbers of IFN-gamma secreting cells, compared with 
      other M nonapeptides and one irrelevant nonapeptide. Bioinformatics analysis 
      showed that the former is an H-2Kd-restricted CTL epitope, and the latter is an 
      H-2Dd-restricted CTL epitope. Multiple amino acid sequence alignment among 
      different PRRSV M sequences submitted to GenBank indicated that these two CTL 
      epitopes are strongly conserved, and they should therefore be considered for 
      further research on the mechanisms of cellular immune responses to PRRSV.
FAU - Zhang, Weijun
AU  - Zhang W
AD  - The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State 
      Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
FAU - Lin, Yan
AU  - Lin Y
FAU - Bai, Yu
AU  - Bai Y
FAU - Tong, Tiegang
AU  - Tong T
FAU - Wang, Qun
AU  - Wang Q
FAU - Liu, Nihong
AU  - Liu N
FAU - Liu, Guangliang
AU  - Liu G
FAU - Xiao, Yihong
AU  - Xiao Y
FAU - Yang, Tao
AU  - Yang T
FAU - Bu, Zhigao
AU  - Bu Z
FAU - Tong, Guangzhi
AU  - Tong G
FAU - Wu, Donglai
AU  - Wu D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110530
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Drug Carriers)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Drug Carriers/administration & dosage
MH  - *Epitope Mapping
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Female
MH  - Genetic Vectors
MH  - Interferon-gamma/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Vaccination/methods
MH  - Vaccinia virus/genetics
MH  - Viral Matrix Proteins/*immunology
MH  - Viral Vaccines/administration & dosage/immunology
PMC - PMC3126774
EDAT- 2011/05/31 06:00
MHDA- 2011/09/14 06:00
PMCR- 2011/05/30
CRDT- 2011/05/31 06:00
PHST- 2010/12/12 00:00 [received]
PHST- 2011/05/30 00:00 [accepted]
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/09/14 06:00 [medline]
PHST- 2011/05/30 00:00 [pmc-release]
AID - 1743-422X-8-263 [pii]
AID - 10.1186/1743-422X-8-263 [doi]
PST - epublish
SO  - Virol J. 2011 May 30;8:263. doi: 10.1186/1743-422X-8-263.