PMID- 21619721
OWN - NLM
STAT- MEDLINE
DCOM- 20110919
LR  - 20191210
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 35
IP  - 5
DP  - 2011 Jun
TI  - Analysis of 3,4-methylenedioxymetamphetamine: whole blood versus dried blood 
      spots.
PG  - 269-73
AB  - Analysis of dried blood spots is an increasingly accepted method in therapeutic 
      drug monitoring, whereas its application by analogy to forensic samples has not 
      been studied in detail. Therefore, we investigated whether determination of 
      3,4-methylenedioxymethamphetamine (MDMA) and its main metabolite 
      3,4-methylendioxyamphetamine (MDA) from dried blood spots (DBS) is as reliable as 
      that from whole blood specimens. Analysis was performed by liquid 
      chromatography-tandem mass spectrometry following liquid-liquid extraction of 
      blood and corresponding DBS samples from 20 volunteers participating in a 
      controlled driving experiment under the influence of MDMA. The assay was checked 
      for carryover, ion suppression/enhancement, linearity of response, lower limits 
      of detection (LLOD) and quantitation, extraction efficiency and the within-run 
      and between-run assay imprecision for both whole blood and DBS. The LLODs were 
      2.0 and 1.6 ng/mL for MDMA in whole blood and DBS, respectively, using a volume 
      of 100 muL. LLODs of MDA were determined to be 0.25 ng/mL in whole blood specimens 
      and 0.12 ng/mL in DBS. Extraction efficiency and imprecision did not differ 
      significantly between the two methods for both MDMA and MDA. The mean 
      concentration ratio of corresponding whole blood and DBS samples, t-test, and the 
      Bland-Altman difference plot were used to test hypothesis of equality. 
      Statistical analyses revealed that methods did not significantly differ for MDMA 
      or MDA. Thus, DBS analysis has potential as a precise and inexpensive alternative 
      to whole blood analysis of MDMA.
FAU - Jantos, Ricarda
AU  - Jantos R
AD  - Institute of Legal Medicine and Traffic Medicine, University Hospital, 
      Heidelberg, Germany. ricarda.jantos@med.uni-heidelberg.de
FAU - Veldstra, Janet L
AU  - Veldstra JL
FAU - Mattern, Rainer
AU  - Mattern R
FAU - Brookhuis, Karel A
AU  - Brookhuis KA
FAU - Skopp, Gisela
AU  - Skopp G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Hallucinogens)
RN  - 0 (Illicit Drugs)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Blood Chemical Analysis/methods
MH  - Hallucinogens/*analysis
MH  - Humans
MH  - Illicit Drugs/*analysis
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*blood
MH  - Substance Abuse Detection/*methods
EDAT- 2011/05/31 06:00
MHDA- 2011/09/20 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/09/20 06:00 [medline]
AID - 10.1093/anatox/35.5.269 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2011 Jun;35(5):269-73. doi: 10.1093/anatox/35.5.269.