PMID- 21619723
OWN - NLM
STAT- MEDLINE
DCOM- 20110919
LR  - 20201209
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 35
IP  - 5
DP  - 2011 Jun
TI  - Drug screening of whole blood by ultra-performance liquid chromatography-tandem 
      mass spectrometry.
PG  - 280-93
AB  - An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) 
      method for screening of drugs in whole blood has been developed and validated. 
      Samples were prepared by supported liquid-liquid extraction on ChemElute((R)) 
      columns with ethyl acetate/heptane (4:1). LC separation was achieved with an 
      Acquity HSS T3-column (2.1 100 mm, 1.8-mum particle). Mass detection was performed 
      by positive ion mode electrospray MS-MS and included the following 
      drugs/metabolites: morphine, codeine, ethyl morphine, oxycodone, buprenorphine, 
      methadone, cocaine, methylphenidate, amphetamine, methamphetamine, 
      3,4-methylenedioxymethamphetamine (MDMA), Delta(9)-tetrahydrocannabinol (THC), 
      fentanyl, alprazolam, bromazepam, clonazepam, diazepam, nordiazepam, 
      3-OH-diazepam, fenazepam, flunitrazepam, lorazepam, nitrazepam, oxazepam, 
      zopiclone, zolpidem, carisoprodol, and meprobamate. The cycle time was 9 min, and 
      within- and between-day relative coefficients of variation varied from 1% to 33% 
      and 2% to 58%, respectively. Extraction recoveries from whole blood were > 50% 
      except for morphine and THC. The limit of quantitation was 0.1 to 521 ng/mL, 
      depending on the drug.
FAU - Oiestad, Elisabeth Leere
AU  - Oiestad EL
AD  - Norwegian Institute of Public Health, Division of Forensic Toxicology and Drug 
      Abuse, Oslo, Norway. elisabeth.oiestad@fhi.no
FAU - Johansen, Unni
AU  - Johansen U
FAU - Oiestad, Ase Marit Leere
AU  - Oiestad AM
FAU - Christophersen, Asbjorg Solberg
AU  - Christophersen AS
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Illicit Drugs)
RN  - 40D3SCR4GZ (Buprenorphine)
RN  - 76I7G6D29C (Morphine)
RN  - 7J8897W37S (Dronabinol)
RN  - CD35PMG570 (Oxycodone)
RN  - I5Y540LHVR (Cocaine)
RN  - RWO67D87EU (Ethylmorphine)
RN  - UC6VBE7V1Z (Methadone)
RN  - UX6OWY2V7J (Codeine)
SB  - IM
MH  - Buprenorphine/blood
MH  - Chromatography, Liquid
MH  - Cocaine/blood
MH  - Codeine/blood
MH  - Dronabinol/blood
MH  - Ethylmorphine/blood
MH  - Humans
MH  - Illicit Drugs/*blood
MH  - Methadone/blood
MH  - Morphine/blood
MH  - Oxycodone/metabolism
MH  - Substance Abuse Detection/*methods
MH  - Substance-Related Disorders/blood
MH  - Tandem Mass Spectrometry
EDAT- 2011/05/31 06:00
MHDA- 2011/09/20 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/09/20 06:00 [medline]
AID - 10.1093/anatox/35.5.280 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2011 Jun;35(5):280-93. doi: 10.1093/anatox/35.5.280.