PMID- 21620821
OWN - NLM
STAT- MEDLINE
DCOM- 20111025
LR  - 20110704
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 666
IP  - 1-3
DP  - 2011 Sep
TI  - Hyperthermia conditioned astrocyte-cultured medium protects neurons from ischemic 
      injury by the up-regulation of HIF-1 alpha and the increased anti-apoptotic 
      ability.
PG  - 19-25
LID - 10.1016/j.ejphar.2011.05.018 [doi]
AB  - It has been demonstrated that conditioned medium from astrocytes challenged by in 
      vitro ischemia (oxygen-glucose deprivation, OGD) improved neuronal survival. In 
      addition, preconditioning stimuli can be cross-tolerant, safeguarding against 
      other types of injury. We therefore hypothesized that hyperthermia-conditioned 
      astrocyte-cultured medium (ACM) might also have protective effect on neurons 
      against ischemic injury. The cultured-media, named 38ACM and 40ACM respectively, 
      were collected after astrocytes had been incubated at 38  degrees C or 40  degrees C for 6h, 
      followed by incubation at 37  degrees C for 24h. It was found that ischemia for 6h 
      induced a significant reduction in the number of neuronal cells and 
      cell-viability, and an increase in lactate dehydrogenase (LDH) release and the 
      percentage of apoptotic nuclei in neurons. Pre-treatment with 38ACM or 40ACM for 
      24h significantly diminished ischemia injury, enhanced cell viability, reduced 
      LDH release and reversed apoptosis. Western blot analysis showed that treatment 
      with 38ACM or 40ACM for 24h led to a significant increase in hypoxia-inducible 
      factor-1 (HIF-1) alpha expression. The EMSA demonstrated that the ACM increased 
      the binding activity of HIF-1 in ischemic neurons. The data implied that 
      hyperthermia-conditioned ACM protects neurons from ischemic injury by 
      up-regulating HIF-1 alpha, and the increased binding activity of HIF-1 and 
      anti-apoptotic ability.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Du, Fang
AU  - Du F
AD  - School of Biomedical Sciences, Faculty of Medicine, The Chinese University of 
      Hong Kong, Shatin, NT, Hong Kong.
FAU - Wu, Xiao Mei
AU  - Wu XM
FAU - Gong, Qi
AU  - Gong Q
FAU - He, Xuan
AU  - He X
FAU - Ke, Ya
AU  - Ke Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110523
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 11096-26-7 (Erythropoietin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Astrocytes/*cytology
MH  - Brain Ischemia/metabolism/*pathology
MH  - Cell Hypoxia/genetics
MH  - Cell Survival/drug effects
MH  - Culture Media, Conditioned/pharmacology
MH  - Erythropoietin/genetics/metabolism
MH  - *Hyperthermia, Induced
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Ischemic Preconditioning
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mice
MH  - Neurons/*drug effects/enzymology/metabolism/pathology
MH  - Response Elements/genetics
MH  - Up-Regulation/*drug effects
EDAT- 2011/05/31 06:00
MHDA- 2011/10/26 06:00
CRDT- 2011/05/31 06:00
PHST- 2010/12/27 00:00 [received]
PHST- 2011/04/20 00:00 [revised]
PHST- 2011/05/03 00:00 [accepted]
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/10/26 06:00 [medline]
AID - S0014-2999(11)00548-6 [pii]
AID - 10.1016/j.ejphar.2011.05.018 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2011 Sep;666(1-3):19-25. doi: 10.1016/j.ejphar.2011.05.018. Epub 
      2011 May 23.