PMID- 21621031
OWN - NLM
STAT- MEDLINE
DCOM- 20110927
LR  - 20191210
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 696
IP  - 1-2
DP  - 2011 Jun 24
TI  - Fast determination of thyroid stimulating hormone in human blood serum without 
      chemical preprocessing by using infrared spectroscopy and least squares support 
      vector machines.
PG  - 47-52
LID - 10.1016/j.aca.2011.04.015 [doi]
AB  - The least squares support vector machines (LS-SVM) was used to model infrared 
      spectral data for TSH hormone secreted by thyroid, which regulates the basal 
      metabolic rate. This model was used for direct estimation of the content of TSH 
      in blood serum samples, and the results were comparable with those obtained with 
      the conventional analytical method based on chemoluminescence methodology. 
      Excellent agreement was observed between the conventional method and the newly 
      developed calibration model based in analysis of spectral data with LS-SVM. The 
      latter has clear advantages, because it is fast and requires no reagent once the 
      measurements were done directly in the serum by using a simple mid-infrared 
      spectrometer in the ATR mode. An important advantage observed in this calibration 
      method based on LS-SVM is the remarkable capacity to avoid overfitting in the 
      model-building step, that is, the developed method is highly robust.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Mello, Cesar
AU  - Mello C
AD  - Centre of environmental Sciences, University of Mogi das Cruzes, SP, Brazil. 
      camello@umc.br
FAU - Marangoni, Antonio
AU  - Marangoni A
FAU - Poppi, Ronei
AU  - Poppi R
FAU - Noda, Isao
AU  - Noda I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110420
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 9002-71-5 (Thyrotropin)
SB  - IM
MH  - Artificial Intelligence
MH  - Humans
MH  - Least-Squares Analysis
MH  - Reproducibility of Results
MH  - Serum/chemistry
MH  - Spectroscopy, Fourier Transform Infrared/economics/*methods
MH  - Thyrotropin/*blood
MH  - Time Factors
EDAT- 2011/05/31 06:00
MHDA- 2011/09/29 06:00
CRDT- 2011/05/31 06:00
PHST- 2010/09/29 00:00 [received]
PHST- 2010/12/20 00:00 [revised]
PHST- 2011/04/12 00:00 [accepted]
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - S0003-2670(11)00537-X [pii]
AID - 10.1016/j.aca.2011.04.015 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2011 Jun 24;696(1-2):47-52. doi: 10.1016/j.aca.2011.04.015. Epub 
      2011 Apr 20.