PMID- 21621213
OWN - NLM
STAT- MEDLINE
DCOM- 20110916
LR  - 20110624
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1218
IP  - 28
DP  - 2011 Jul 15
TI  - Simple, high throughput ultra-high performance liquid chromatography/tandem mass 
      spectrometry trace analysis of perfluorinated alkylated substances in food of 
      animal origin: milk and fish.
PG  - 4312-21
LID - 10.1016/j.chroma.2011.04.061 [doi]
AB  - The present study documents development and validation of a novel approach for 
      determination of 23 perfluorinated alkylated substances (PFASs) in food of animal 
      origin represented by milk and fish. The list of target analytes comprises four 
      classes of PFASs, both ionic and non-ionic: 11 perfluorocarboxylic acids (PFCAs), 
      4 perfluorosulphonic acids (PFSAs), 5 perfluorosulphonamides (FOSAs) and 3 
      perfluorophosphonic acids (PFPAs). Fast sample preparation procedure is based on 
      an extraction of target analytes with acetonitrile (MeCN) and their transfer 
      (supported by inorganic salts and acidification) into the organic phase. Removing 
      of matrix co-extracts by a simple dispersive solid phase extraction (SPE) 
      employing ENVI-Carb and C18 sorbents is followed by an efficient sample 
      pre-concentration performed by acetonitrile evaporation and subsequent dilution 
      of residue in a small volume of methanol (matrix equivalent in the final extracts 
      was 16 and 8 g mL(-1), for milk and fish respectively). Using modern 
      instrumentation consisting of ultra-high performance liquid chromatography 
      (UHPLC) hyphenated with a tandem mass spectrometer (MS/MS), limits of 
      quantification (LOQs) as low as 0.001-0.006 mug kg(-1) for milk and 0.002-0.013 mug 
      kg(-1) for fish can be achieved. Under these conditions, a wide spectrum of 
      PFASs, including minor representatives, can be determined which enables 
      collecting data required for human exposure studies. The pilot study employing 
      the new method for examination of milk and canned fish samples was realized. 
      Whereas in majority of canned fish products a wide spectrum of PFCAs, 
      perfluorooctanesulphonic acid (PFOS) and perfluoro-1-octanesulphonamide (PFOSA) 
      was detected, only in a few milk samples very low concentrations (LOQ levels) of 
      PFOS and perfluorooctansulphonic acid (PFDS) were found.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Lacina, Ondrej
AU  - Lacina O
AD  - Institute of Chemical Technology, Faculty of Food and Biochemical Technology, 
      Department of Food Chemistry and Analysis, Prague, Czech Republic.
FAU - Hradkova, Petra
AU  - Hradkova P
FAU - Pulkrabova, Jana
AU  - Pulkrabova J
FAU - Hajslova, Jana
AU  - Hajslova J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110507
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (Fluorocarbons)
SB  - IM
MH  - Alkanesulfonic Acids/analysis
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Dairy Products/*analysis
MH  - *Fishes
MH  - Fluorocarbons/*analysis
MH  - Food, Preserved/analysis
MH  - High-Throughput Screening Assays/*methods
MH  - Meat/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/methods
EDAT- 2011/05/31 06:00
MHDA- 2011/09/17 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/01/04 00:00 [received]
PHST- 2011/04/11 00:00 [revised]
PHST- 2011/04/24 00:00 [accepted]
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/09/17 06:00 [medline]
AID - S0021-9673(11)00571-1 [pii]
AID - 10.1016/j.chroma.2011.04.061 [doi]
PST - ppublish
SO  - J Chromatogr A. 2011 Jul 15;1218(28):4312-21. doi: 10.1016/j.chroma.2011.04.061. 
      Epub 2011 May 7.