PMID- 21621594
OWN - NLM
STAT- MEDLINE
DCOM- 20110906
LR  - 20141120
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 205
IP  - 1
DP  - 2011 Aug 10
TI  - Novel gene markers of immunosuppressive chemicals in mouse lymph node assay.
PG  - 79-85
LID - 10.1016/j.toxlet.2011.05.1017 [doi]
AB  - The murine local lymph node assay (LLNA) is an immunologically based test of the 
      sensitizing potential of immunotoxicants, but also evaluates immunosuppressive 
      potential with good sensitivity and specificity. We conducted the LLNA with 
      calcineurin inhibitors (tacrolimus and cyclosporin A), antimetabolites 
      (methotrexate and azathioprine), steroids (dexamethasone and prednisolone), and 
      an alkylator (cyclophosphamide). We performed a comprehensive analysis of results 
      of gene expression in lymph nodes obtained in the LLNA using a highly sensitive 
      DNA chip, 3D-Gene, and the quantitative reverse transcription-polymerase chain 
      reaction (qPCR). Zfp459 expression increased in all animals treated with 
      immunosuppressive chemicals. Ltf, Cbll1 and Lias expression changed specifically 
      in response to the calcineurin inhibitors, Fmo2 and 9630033F20Rik in response to 
      the antimetabolites, Krt8, Gjb1, Hmha1 and Sfrs7 in response to the steroids, and 
      Gbp1 and Mup5 in response to the alkylator. Therefore, these genes were 
      considered novel markers for immunosuppression and their expression could be used 
      to evaluate the mechanism of action of immunosuppressive chemicals.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Oshida, Keiyu
AU  - Oshida K
AD  - Toxicology and Pharmacokinetics Laboratories, Pharmaceutical Research 
      Laboratories, Toray Industries, Inc., 6-10-1, Tebiro, Kamakura, Kanagawa 
      248-8555, Japan.
FAU - Maeda, Akihisa
AU  - Maeda A
FAU - Kitsukawa, Mika
AU  - Kitsukawa M
FAU - Suga, Sachiko
AU  - Suga S
FAU - Iwano, Shunsuke
AU  - Iwano S
FAU - Miyoshi, Tomoya
AU  - Miyoshi T
FAU - Miyamoto, Yohei
AU  - Miyamoto Y
LA  - eng
PT  - Journal Article
DEP - 20110520
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Alkylating Agents)
RN  - 0 (Antimetabolites)
RN  - 0 (Calcineurin Inhibitors)
RN  - 0 (Genetic Markers)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Steroids)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Alkylating Agents/pharmacology
MH  - Animals
MH  - Antimetabolites/pharmacology
MH  - Body Weight/drug effects
MH  - Calcineurin Inhibitors
MH  - Female
MH  - Gene Expression/drug effects
MH  - Genetic Markers
MH  - Immunosuppressive Agents/*pharmacology
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred CBA
MH  - Oligonucleotide Array Sequence Analysis
MH  - Organ Size/drug effects
MH  - RNA/biosynthesis/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium Dodecyl Sulfate/toxicity
MH  - Steroids/pharmacology
EDAT- 2011/05/31 06:00
MHDA- 2011/09/07 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/02/02 00:00 [received]
PHST- 2011/05/11 00:00 [revised]
PHST- 2011/05/12 00:00 [accepted]
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/09/07 06:00 [medline]
AID - S0378-4274(11)01217-3 [pii]
AID - 10.1016/j.toxlet.2011.05.1017 [doi]
PST - ppublish
SO  - Toxicol Lett. 2011 Aug 10;205(1):79-85. doi: 10.1016/j.toxlet.2011.05.1017. Epub 
      2011 May 20.