PMID- 2162166
OWN - NLM
STAT- MEDLINE
DCOM- 19900716
LR  - 20131121
IS  - 0158-5231 (Print)
IS  - 0158-5231 (Linking)
VI  - 20
IP  - 4
DP  - 1990
TI  - Glucocorticoids regulate expression of the v-mycOK10 oncogene in a murine 
      retroviral vector with chimeric MoMuLV-MMTV LTRs.
PG  - 669-79
AB  - A murine retrovirus which expresses the v-mycOK10 oncogene under the control of 
      the dexamethasone-regulatable mouse mammary tumor virus (MMTV) promoter has been 
      constructed. In this vector, denoted pMImyc, the Moloney Murine leukemia virus 
      (MoMuLV) sequences required for virus replication, integration and packaging were 
      kept, while all the elements for transcription regulation were derived from the 
      MMTV long terminal repeat (LTR). After transfection of NIH 3T3 fibroblasts with 
      this construct, a cell line was isolated in which the level of v-myc RNAs were 
      increased 60 fold by dexamethasone. Kinetic studies showed that this induction 
      can be maintained for up to 12 hours of hormone treatment. After infection with 
      MoMuLV as a helper virus, and in the presence of dexamethasone, the production of 
      pMImyc RNA, estimated by slot blot analysis, was equivalent to about 10(3) viral 
      particles/ml.
FAU - Jacquemin-Sablon, H
AU  - Jacquemin-Sablon H
AD  - Molecular Biology and Virology Laboratory, Salk Institute, San Diego.
FAU - Bogenberger, J
AU  - Bogenberger J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Biochem Int
JT  - Biochemistry international
JID - 8100311
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Line
MH  - Chimera/genetics
MH  - Dexamethasone/*pharmacology
MH  - Fibroblasts
MH  - Gene Expression Regulation, Neoplastic
MH  - Genetic Vectors
MH  - Kinetics
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Moloney murine leukemia virus/*genetics
MH  - *Oncogenes
MH  - Promoter Regions, Genetic
MH  - Repetitive Sequences, Nucleic Acid
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
PST - ppublish
SO  - Biochem Int. 1990;20(4):669-79.