PMID- 21621872
OWN - NLM
STAT- MEDLINE
DCOM- 20111227
LR  - 20110829
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 216
IP  - 10
DP  - 2011 Oct
TI  - Kaempferol from Semen cuscutae attenuates the immune function of dendritic cells.
PG  - 1103-9
LID - 10.1016/j.imbio.2011.05.002 [doi]
AB  - Dendritic cells (DCs) are the critical leukocytes in regulating immune responses. 
      Accordingly, DCs are the major target in the development of immunomodulators. In 
      this study, we examined the effect of Semen cuscutae (SC), an important 
      traditional Chinese medicine, on mouse bone marrow-derived DCs. We found that the 
      n-butanol and methanol extracts of SC significantly suppressed LPS-stimulated DC 
      activation. Several flavonoids were verified in the extracts using HPLC, and then 
      kaempferol was identified as the major flavonoid in the methanol fraction of SC. 
      Kaempferol was able to reduce cytokines and chemokines produced by LPS-stimulated 
      DCs, and this reduction was not due to its cytotoxicity on DCs. In addition, DC 
      maturation was impaired by kaempferol. Furthermore, kaempferol abrogated the 
      ability of LPS-stimulated DCs to promote Ag-specific T cell activation, both in 
      vitro and in vivo. Thus, we show for the first time that SC exhibits an 
      immunosuppressive effect on DCs and that the active ingredient kaempferol 
      attenuates DC function, which suggests that kaempferol has potential in the 
      treatment of chronic inflammatory and autoimmune diseases.
CI  - Copyright (c) 2011 Elsevier GmbH. All rights reserved.
FAU - Lin, Ming-Kuem
AU  - Lin MK
AD  - School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China 
      Medical University, Taichung, Taiwan.
FAU - Yu, Yen-Ling
AU  - Yu YL
FAU - Chen, Kai-Chieh
AU  - Chen KC
FAU - Chang, Wen-Te
AU  - Chang WT
FAU - Lee, Meng-Shiou
AU  - Lee MS
FAU - Yang, Meng-Ja
AU  - Yang MJ
FAU - Cheng, Hsin-Chung
AU  - Cheng HC
FAU - Liu, Chien-Heng
AU  - Liu CH
FAU - Chen, Dz-Chi
AU  - Chen DzC
FAU - Chu, Ching-Liang
AU  - Chu CL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110531
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Kaempferols)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Cell Aggregation/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Cuscuta/*chemistry
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/cytology/*drug effects/*immunology
MH  - Drugs, Chinese Herbal/*pharmacology/toxicity
MH  - Kaempferols/*pharmacology/toxicity
MH  - Lipopolysaccharides/immunology
MH  - Lymphocyte Activation/drug effects/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes/drug effects/immunology
EDAT- 2011/05/31 06:00
MHDA- 2011/12/28 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/01/09 00:00 [received]
PHST- 2011/04/12 00:00 [revised]
PHST- 2011/05/01 00:00 [accepted]
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/12/28 06:00 [medline]
AID - S0171-2985(11)00094-5 [pii]
AID - 10.1016/j.imbio.2011.05.002 [doi]
PST - ppublish
SO  - Immunobiology. 2011 Oct;216(10):1103-9. doi: 10.1016/j.imbio.2011.05.002. Epub 
      2011 May 31.