PMID- 21622127
OWN - NLM
STAT- MEDLINE
DCOM- 20111010
LR  - 20220224
IS  - 1945-0508 (Electronic)
IS  - 1945-0494 (Linking)
VI  - 3
IP  - 4
DP  - 2011 Jun 1
TI  - Developmental patterns of PPAR and RXR gene expression during spermatogenesis.
PG  - 1209-20
AB  - Members of the family of nuclear receptors that include peroxisome 
      proliferator-activated receptors (PPARs) and retinoid X receptors (RXRs) are 
      important mediators of selective gene activation during development and cellular 
      differentiation. In this study, developmentally-specific PPAR and RXR patterns of 
      expression that occur in somatic and germ cell populations in the testis were 
      determined using quantative real-time PCR (qRT-PCR) studies on RNAs that were 
      isolated from StaPut purified mouse germ cells and primary rat Sertoli cells. 
      These qRT-PCR studies indicate that transcripts encoding the PPAR-Alpha (alpha), 
      -Beta (beta), and -Gamma (gamma) and RXR -Alpha (alpha), -Beta (beta), and -Gamma (gamma) are 
      developmentally expressed in both differentiating germ and Sertoli cells. In 
      further experiments aimed at deciphering the physiological role that PPAR-Gamma 
      (gamma) plays in Sertoli cells, 15-day primary rat Sertoli cells were infected with 
      recombinant adenoviral vectors containing PPAR-Gamma (gamma) cDNA and PPAR-Gamma (gamma) 
      RNAi constructs. Affymetrix microarray analysis and qRT-PCR validation studies 
      using total RNA isolated from these transfected cells indicated that PPAR-Gamma 
      regulates the pattern of expression of key lipid metabolic genes in Sertoli 
      cells.
FAU - Thomas, Kelwyn
AU  - Thomas K
AD  - Department of Anatomy and Neurobiology, Morehouse School of Medicine, Atlanta, GA 
      30310-1495, USA. kethomas@msm.edu
FAU - Sung, Dae-Yong
AU  - Sung DY
FAU - Chen, Xing
AU  - Chen X
FAU - Thompson, Winston
AU  - Thompson W
FAU - Chen, Y Eugene
AU  - Chen YE
FAU - McCarrey, John
AU  - McCarrey J
FAU - Walker, William
AU  - Walker W
FAU - Griswold, Michael
AU  - Griswold M
LA  - eng
GR  - G12 RR003034/RR/NCRR NIH HHS/United States
GR  - S06 GM008248/GM/NIGMS NIH HHS/United States
GR  - HD41749/HD/NICHD NIH HHS/United States
GR  - RR03034/RR/NCRR NIH HHS/United States
GR  - 806GM08248/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110601
PL  - Singapore
TA  - Front Biosci (Elite Ed)
JT  - Frontiers in bioscience (Elite edition)
JID - 101485240
RN  - 0 (DNA Primers)
RN  - 0 (Peroxisome Proliferator-Activated Receptors)
RN  - 0 (Retinoid X Receptors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - DNA Primers
MH  - *Gene Expression Regulation, Developmental
MH  - Male
MH  - Mice
MH  - Oligonucleotide Array Sequence Analysis
MH  - Peroxisome Proliferator-Activated Receptors/*genetics
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinoid X Receptors/*genetics
MH  - Spermatogenesis/*genetics
EDAT- 2011/05/31 06:00
MHDA- 2011/10/11 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/10/11 06:00 [medline]
AID - 324 [pii]
AID - 10.2741/E324 [doi]
PST - epublish
SO  - Front Biosci (Elite Ed). 2011 Jun 1;3(4):1209-20. doi: 10.2741/E324.