PMID- 21622193
OWN - NLM
STAT- MEDLINE
DCOM- 20111003
LR  - 20220225
IS  - 2768-6698 (Electronic)
IS  - 2768-6698 (Linking)
VI  - 16
IP  - 7
DP  - 2011 Jun 1
TI  - Experimental study of 99mTc-depreotide preparation and its affinity with A549 
      cell.
PG  - 2527-39
AB  - The (99m)Tc-labeled agent, ((99m)TcO)depreotide, has received regulatory approval 
      in the United States and Europe for use in the detection of cancer. It is 
      essential to establish a simple and reliable method of direct radiolabeling of 
      (99m)Tc-depreotide and to investigate its specific receptor binding properties 
      with human non small cell lung cancer (NSCLC) A549 cell in vitro. So we made some 
      researches as follow: Depreotide was labeled with (99m)Tc using SnCl2 as a 
      reductant. Labeling efficiencies at different pH values and temperatures were 
      compared. Radioreceptor assay was used to observe the uptake kinetics, stagnation 
      and retention half time of (99m)Tc-depreotide in A549 cells. As the results of 
      the investigation ,many facts is shown below: The labeling rate of pH 6.0 group 
      was higher than that of pH 5.0 and pH7.0 groups. The labeling rate decreased when 
      temperature increased from 15  degrees C to 50  degrees C. The uptake rate increased with rising 
      temperature, and the maximum uptake was observed at 60 min at 37  degrees C. The cleaning 
      curves were similar at different temperatures, and the half cleaning time at 37 
       degrees C was 48 min. The results showed that the optimal conditions for labeling 
      depreotide with (99m)Tc was found to be below 15  degrees C at a pH lower than 6.0. 
      Furthermore, at 37  degrees C, (99m)Tc-depreotid may have the potential as an ideal 
      imaging agent for somatostatin receptors.
FAU - Xia, Wei
AU  - Xia W
AD  - Department of Nuclear Medicine, 10th People's Hospital, Tongji University of 
      Medcine School, Shanghai 200072, China.
FAU - Zhang, Xiaoping
AU  - Zhang X
FAU - Yu, Fei
AU  - Yu F
FAU - Wang, Peijun
AU  - Wang P
FAU - Cai, Haidong
AU  - Cai H
FAU - Hou, Renhua
AU  - Hou R
FAU - Wang, Guoyu
AU  - Wang G
FAU - Yuan, Xueyu
AU  - Yuan X
FAU - Lv, Zhongwei
AU  - Lv Z
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110601
PL  - Singapore
TA  - Front Biosci (Landmark Ed)
JT  - Frontiers in bioscience (Landmark edition)
JID - 101612996
RN  - 0 (Organotechnetium Compounds)
RN  - 0 (Radiopharmaceuticals)
RN  - 0 (Receptors, Somatostatin)
RN  - 51110-01-1 (Somatostatin)
RN  - 9M48M2SF02 (technetium Tc 99m depreotide)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*diagnostic imaging/metabolism
MH  - Cell Line, Tumor
MH  - Chromatography, High Pressure Liquid
MH  - Drug Stability
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lung Neoplasms/*diagnostic imaging/metabolism
MH  - Organotechnetium Compounds/chemical synthesis/*isolation & purification
MH  - Radionuclide Imaging
MH  - Radiopharmaceuticals/chemical synthesis/*isolation & purification
MH  - Receptors, Somatostatin/metabolism
MH  - Somatostatin/*analogs & derivatives/chemical synthesis/isolation & purification
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Temperature
EDAT- 2011/05/31 06:00
MHDA- 2011/10/04 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/10/04 06:00 [medline]
AID - 3870 [pii]
AID - 10.2741/3870 [doi]
PST - epublish
SO  - Front Biosci (Landmark Ed). 2011 Jun 1;16(7):2527-39. doi: 10.2741/3870.