PMID- 21622880
OWN - NLM
STAT- MEDLINE
DCOM- 20120126
LR  - 20220409
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 89
IP  - 10
DP  - 2011 Oct
TI  - Mannan oligosaccharide modulates gene expression profile in pigs experimentally 
      infected with porcine reproductive and respiratory syndrome virus.
PG  - 3016-29
LID - 10.2527/jas.2010-3366 [doi]
AB  - This study characterized gene expression in peripheral blood mononuclear cells 
      (PBMC) and bronchoalveolar lavage fluid (BALF) cells from control- or mannan 
      oligosaccharide (MOS)-fed pigs with or without porcine reproductive and 
      respiratory syndrome virus (PRRSV) at d 7 postinfection (PI). Weaned pigs (3 wk 
      old) fed 0 or 0.2% MOS (Bio-Mos) diets were intranasally inoculated with PRRSV or 
      a sterile medium at 5 wk of age. Total RNA (3 pigs/treatment) was extracted from 
      cells. Double-stranded cDNA was amplified, labeled, and further hybridized to the 
      Affymetrix GeneChip Porcine Genome Array consisting of 23,937 probe sets 
      representing 20,201 genes. Microarray data were analyzed in R using packages from 
      the Bioconductor project. Differential gene expression was tested by fitting a 
      mixed linear model equivalent to a 2 x 2 factorial ANOVA using the limma package. 
      Dietary MOS and PRRSV changed the expression of thousands of probe sets in PBMC 
      and BALF cells (P < 0.05). The MOS x PRRSV interaction altered the expression of 
      more nonimmune probe sets in PBMC (977 up, 1,128 down) than in BALF cells (117 
      up, 78 down). The MOS x PRRSV interaction (P < 0.05) for immune probe sets in 
      PBMC affected genes encoding key inflammatory mediators. In uninfected pigs, gene 
      expression of IL-1alpha, IL-6, myeloid differentiation factor 88, Toll-like receptor 
      (TLR) 4, major histocompatibility complex (MHC) II, and dead box polypeptide 58 
      increased in PBMC of MOS-fed pigs (P < 0.05). This suggests that MOS enhances 
      disease resistance in pigs and supports the fact that MOS induced a rapid 
      increase in leukocytes at d 3 and 7 PI. Within infected pigs, however, MOS 
      reduced the expression of IL-1beta, IL-6, IL-8, macrophage inflammatory protein 
      (MIP)-1alpha, MIP-1beta, monocyte chemotactic protein (MCP)-1, and TLR4 genes in PBMC (P 
      < 0.05). This finding may explain why fever was ameliorated in infected pigs fed 
      MOS by d 7 PI. The expression of IL-1beta, IL-6, MIP-1beta, MCP-1, and TLR4 genes was 
      confirmed by quantitative real-time reverse-transcription PCR. In BALF cells of 
      infected pigs, MOS reduced the gene expression of TLR4, MHCII, and molecules 
      associated with the complement system, but increased the gene expression of MHCI. 
      In short, MOS regulated the expression of nonimmune and immune genes in pig 
      leukocytes, perhaps providing benefits by enhancing the immune responses of the 
      pigs to an infection, while preventing overstimulation of the immune system.
FAU - Che, T M
AU  - Che TM
AD  - Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA. 
      chetung2@illinois.edu
FAU - Johnson, R W
AU  - Johnson RW
FAU - Kelley, K W
AU  - Kelley KW
FAU - Van Alstine, W G
AU  - Van Alstine WG
FAU - Dawson, K A
AU  - Dawson KA
FAU - Moran, C A
AU  - Moran CA
FAU - Pettigrew, J E
AU  - Pettigrew JE
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20110527
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Mannans)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Diet/veterinary
MH  - Gene Expression Profiling/*veterinary
MH  - Gene Expression Regulation/drug effects
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - Mannans/*pharmacology
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Protein Array Analysis
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
EDAT- 2011/05/31 06:00
MHDA- 2012/01/27 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2012/01/27 06:00 [medline]
AID - jas.2010-3366 [pii]
AID - 10.2527/jas.2010-3366 [doi]
PST - ppublish
SO  - J Anim Sci. 2011 Oct;89(10):3016-29. doi: 10.2527/jas.2010-3366. Epub 2011 May 
      27.