PMID- 21622977
OWN - NLM
STAT- MEDLINE
DCOM- 20111220
LR  - 20211020
IS  - 1756-1663 (Electronic)
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 18
IP  - 4
DP  - 2011 Aug
TI  - Sequence capture and next generation resequencing of the MHC region highlights 
      potential transplantation determinants in HLA identical haematopoietic stem cell 
      transplantation.
PG  - 201-10
LID - 10.1093/dnares/dsr008 [doi]
AB  - How cells coordinate the immune system activities is important for potentially 
      life-saving organ or stem cell transplantations. Polymorphic immunoregulatory 
      genes, many of them located in the human major histocompatibility complex, impact 
      the process and assure the proper execution of tolerance-versus-activity 
      mechanisms. In haematopoietic stem cell transplantation, on the basis of fully 
      human leukocyte antigen (HLA)-matched donor-recipient pairs, adverse effects like 
      graft versus leukaemia and graft versus host are observed and difficult to 
      handle. So far, high-resolution HLA typing was performed with Sanger sequencing, 
      but for methodological reasons information on additional immunocompetent major 
      histocompatibility complex loci has not been revealed. Now, we have used 
      microarray sequence capture and targeted enrichment combined with next generation 
      pyrosequencing for 3.5 million base pair human major histocompatibility complex 
      resequencing in a clinical transplant setting and describe 3025 variant single 
      nucleotide polymorphisms, insertions and deletions among recipient and donor in a 
      single sequencing experiment. Taken together, the presented data show that 
      sequence capture and massively parallel pyrosequencing can be used as a new tool 
      for risk assessment in the setting of allogeneic stem cell transplantation.
FAU - Proll, Johannes
AU  - Proll J
AD  - Red Cross Transfusion Service for Upper Austria, Linz. 
      johannes.proell@o.roteskreuz.at
FAU - Danzer, Martin
AU  - Danzer M
FAU - Stabentheiner, Stephanie
AU  - Stabentheiner S
FAU - Niklas, Norbert
AU  - Niklas N
FAU - Hackl, Christa
AU  - Hackl C
FAU - Hofer, Katja
AU  - Hofer K
FAU - Atzmuller, Sabine
AU  - Atzmuller S
FAU - Hufnagl, Peter
AU  - Hufnagl P
FAU - Gully, Christian
AU  - Gully C
FAU - Hauser, Hanns
AU  - Hauser H
FAU - Krieger, Otto
AU  - Krieger O
FAU - Gabriel, Christian
AU  - Gabriel C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110528
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes 
      and genomes
JID - 9423827
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Epitopes/*genetics
MH  - Graft vs Host Disease/genetics
MH  - HLA Antigens/*genetics
MH  - *Hematopoietic Stem Cell Transplantation
MH  - *High-Throughput Nucleotide Sequencing
MH  - Histocompatibility Testing
MH  - Humans
MH  - Polymorphism, Single Nucleotide/genetics
MH  - *Sequence Analysis, DNA
PMC - PMC3158463
EDAT- 2011/05/31 06:00
MHDA- 2011/12/21 06:00
PMCR- 2011/05/28
CRDT- 2011/05/31 06:00
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/12/21 06:00 [medline]
PHST- 2011/05/28 00:00 [pmc-release]
AID - dsr008 [pii]
AID - 10.1093/dnares/dsr008 [doi]
PST - ppublish
SO  - DNA Res. 2011 Aug;18(4):201-10. doi: 10.1093/dnares/dsr008. Epub 2011 May 28.