PMID- 21623202
OWN - NLM
STAT- MEDLINE
DCOM- 20111003
LR  - 20141120
IS  - 1538-7151 (Electronic)
IS  - 0277-1691 (Linking)
VI  - 30
IP  - 4
DP  - 2011 Jul
TI  - Assessing the impact of polysomy-17 on HER2 status and the correlations of HER2 
      status with prognostic variables (ER, PR, p53, Ki-67) in epithelial ovarian 
      cancer: a tissue microarray study using immunohistochemistry and fluorescent in 
      situ hybridization.
PG  - 372-9
LID - 10.1097/PGP.0b013e31820c9ff3 [doi]
AB  - Although HER2 overexpression and Her2 amplification have been noted in breast and 
      a variety of human cancers, we report here for the first time the impact of 
      polysomy-17 on HER2 status and the correlations between HER2 status and other 
      prognostic factors in patients with epithelial ovarian cancers (EOC).We analyzed 
      HER2, estrogen receptor (ER), progesterone receptor (PR), p53, and Ki-67 protein 
      overexpressions by immunohistochemistry (IHC) and determined Her2 gene 
      amplification by fluorescence in situ hybridization (FISH) in 27 tissue 
      microarray samples from EOC patients.We achieved 100% positive concordance (3/3) 
      and 100% negative concordance (19/19) between HER2 testing by IHC and FISH. Both 
      the total Her2 gene copies and FISH scores increased significantly in a stepwise 
      order through the negative, equivocal, and positive HER2 IHC result categories in 
      all 27 cases (P=0.001, P=0.001), and still increased significantly in 18 
      nonpolysomy-17 cases (P=0.007 and 0.013) after the exclusion of 9 polysomy-17 
      cases. HER2 protein expression is inversely correlated with both ER (P=0.002) and 
      PR expressions (P=0.046). Her2 gene amplification is inversely correlated with ER 
      expression (P=0.007) but not with PR expression (P=0.106).This study showed 
      extremely high positive and negative concordances between Her2 FISH and HER2 IHC 
      assays. Polysomy-17 is insufficient for causing a significant impact on the 
      relationship between HER2 testing by IHC and FISH in EOC. ER and PR expressions 
      were inversely correlated with HER2 protein expression. In addition, ER but not 
      PR expression is inversely correlated with Her2 gene amplification.
FAU - Lin, Chih-Kuang
AU  - Lin CK
AD  - Institute of Medicine, Chung Shan Medical University Hospital and School of 
      Medicine, Chung Shan Medical University, Taichung, Taipei, Taiwan.
FAU - Lin, Wea-Lung
AU  - Lin WL
FAU - Chen, Fong-Lin
AU  - Chen FL
FAU - Lee, Ming-Yung
AU  - Lee MY
FAU - Kuo, Jang-Fang
AU  - Kuo JF
FAU - Ruan, Alexandra
AU  - Ruan A
FAU - Tyan, Yeu-Sheng
AU  - Tyan YS
FAU - Chiang, Hung
AU  - Chiang H
FAU - Chou, Ming-Chih
AU  - Chou MC
FAU - Han, Chih-Ping
AU  - Han CP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Gynecol Pathol
JT  - International journal of gynecological pathology : official journal of the 
      International Society of Gynecological Pathologists
JID - 8214845
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Aneuploidy
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/analysis
MH  - Microarray Analysis
MH  - Ovarian Neoplasms/chemistry/*genetics
MH  - Prognosis
MH  - Receptor, ErbB-2/*analysis/genetics
MH  - Receptors, Estrogen/*analysis
MH  - Receptors, Progesterone/*analysis
MH  - Tumor Suppressor Protein p53/analysis
EDAT- 2011/05/31 06:00
MHDA- 2011/10/04 06:00
CRDT- 2011/05/31 06:00
PHST- 2011/05/31 06:00 [entrez]
PHST- 2011/05/31 06:00 [pubmed]
PHST- 2011/10/04 06:00 [medline]
AID - 10.1097/PGP.0b013e31820c9ff3 [doi]
PST - ppublish
SO  - Int J Gynecol Pathol. 2011 Jul;30(4):372-9. doi: 10.1097/PGP.0b013e31820c9ff3.