PMID- 21625177
OWN - NLM
STAT- MEDLINE
DCOM- 20111020
LR  - 20110823
IS  - 1423-0135 (Electronic)
IS  - 1018-1172 (Linking)
VI  - 48
IP  - 5
DP  - 2011
TI  - Characterization of human late outgrowth endothelial progenitor-derived cells 
      under various flow conditions.
PG  - 443-51
LID - 10.1159/000324844 [doi]
AB  - BACKGROUND: Endothelial progenitor-derived cells (EPC) are a cell therapy tool in 
      peripheral arterial disease and for re-endothelialization of bypasses and stents. 
      OBJECTIVE: To assess EPC behavior under flow conditions normally found in vivo. 
      RESULTS: EPC were isolated from human cord blood, cultured on compliant tubes and 
      exposed in an in vitro flow system mimicking hemodynamic environments normally 
      found in medium and large arteries. EPC exposed for 24 h to unidirectional (0.3 +/- 
      0.1 or 6 +/- 3 dynes/cm(2)) shear stress oriented along flow direction, while those 
      exposed to bidirectional shear stress (0.3 +/- 3 dynes/cm(2)) or static conditions 
      had random orientation. Under bidirectional flow, tissue factor (TF) activity and 
      mRNA expression were significantly increased (2.5- and 7.0-fold) compared to 
      static conditions. Under low shear unidirectional flow TF mRNA increased 4.9 +/- 
      0.5-fold. Similar flow-induced increases were observed for TF in mature umbilical 
      vein-derived endothelial cells. Expression of tissue-type plasminogen activator 
      (t-PA), urokinase (u-PA) and monocyte chemotactic protein 1 (MCP1) were reduced 
      by 40-60% in late outgrowth endothelial progenitor-derived cells (LO-EPC) exposed 
      to any flow environment, while MCP1, but not t-PA or u-PA, was decreased in 
      HUVEC. CONCLUSIONS: Flow, in particular bidirectional, modifies the hemostatic 
      balance in LO-EPC with increased TF and decreased plasminogen activator 
      expression.
CI  - Copyright (c) 2011 S. Karger AG, Basel.
FAU - Mazzolai, Lucia
AU  - Mazzolai L
AD  - Division of Vascular Medicine, University Hospital of Lausanne, Fribourg, 
      Switzerland. lucia.mazzolai@chuv.ch
FAU - Bouzourene, Karima
AU  - Bouzourene K
FAU - Hayoz, Daniel
AU  - Hayoz D
FAU - Dignat-George, Francoise
AU  - Dignat-George F
FAU - Liu, Jia Wei
AU  - Liu JW
FAU - Bounameaux, Henri
AU  - Bounameaux H
FAU - Dunoyer-Geindre, Sylvie
AU  - Dunoyer-Geindre S
FAU - Kruithof, Egbert K O
AU  - Kruithof EK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110531
PL  - Switzerland
TA  - J Vasc Res
JT  - Journal of vascular research
JID - 9206092
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Endothelial Cells/*cytology/*physiology
MH  - Fetal Blood/cytology
MH  - Gene Expression/physiology
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Neovascularization, Physiologic/*physiology
MH  - Pulsatile Flow/*physiology
MH  - RNA, Messenger/metabolism
MH  - Stress, Mechanical
MH  - Thromboplastin/genetics/metabolism
MH  - Tissue Plasminogen Activator/genetics/metabolism
MH  - Umbilical Cord/cytology
MH  - Urokinase-Type Plasminogen Activator/genetics/metabolism
EDAT- 2011/06/01 06:00
MHDA- 2011/10/21 06:00
CRDT- 2011/06/01 06:00
PHST- 2010/04/07 00:00 [received]
PHST- 2011/01/01 00:00 [accepted]
PHST- 2011/06/01 06:00 [entrez]
PHST- 2011/06/01 06:00 [pubmed]
PHST- 2011/10/21 06:00 [medline]
AID - 000324844 [pii]
AID - 10.1159/000324844 [doi]
PST - ppublish
SO  - J Vasc Res. 2011;48(5):443-51. doi: 10.1159/000324844. Epub 2011 May 31.