PMID- 21625915
OWN - NLM
STAT- MEDLINE
DCOM- 20120427
LR  - 20220408
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 144
IP  - 1-3
DP  - 2011 Dec
TI  - Effect of boron on osteogenic differentiation of human bone marrow stromal cells.
PG  - 306-15
LID - 10.1007/s12011-011-9094-x [doi]
AB  - Bone marrow stromal cells (BMSCs) have been well established as an ideal source 
      of cell-based therapy for bone tissue engineering applications. Boron (B) is a 
      notable trace element in humans; so far, the effects of boron on the osteogenic 
      differentiation of BMSCs have not been reported. The aim of this study was to 
      evaluate the effects of boron (0, 1, 10,100, and 1,000 ng/ml) on osteogenic 
      differentiation of human BMSCs. In this study, BMSCs proliferation was analyzed 
      by cell counting kit-8 (CCK8) assay, and cell osteogenic differentiation was 
      evaluated by alkaline phosphatase (ALP) activity assay, Von Kossa staining, and 
      real-time PCR. The results indicated that the proliferation of BMSCs was no 
      different from the control group when added with B at the concentration of 1, 10, 
      and 100 ng/ml respectively (P > 0.05); in contrast, 1,000 ng/ml B inhibited the 
      proliferation of BMSCs at days 4, 7, and 14 (P < 0.05). By ALP staining, we 
      discovered that BMSCs treated with 10 and 100 ng/ml B presented a higher ALP 
      activity compared with control (P < 0.05). By real-time PCR, we detected the 
      messenger RNA expression of ALP, osteocalcin, collagen type I, and bone 
      morphogenetic proteins 7 were also increased in 10 and 100 ng/ml B treatment 
      groups (P < 0.05). The calcium depositions were increased in 1 and 10 ng/ml B 
      treatment groups (P < 0.05). Taken all together, it was the first time to report 
      that B could increase osteogenic effect by stimulating osteogenic 
      differentiation-related marker gene synthesis during the proliferation and 
      differentiation phase in human BMSCs and could be a promising approach for 
      enhancing osteogenic capacity of cell-based construction in bone tissue 
      engineering.
FAU - Ying, Xiaozhou
AU  - Ying X
AD  - Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou 
      Medical College, 109 Xue Yuan Xi Road, Wenzhou, 325000, China.
FAU - Cheng, Shaowen
AU  - Cheng S
FAU - Wang, Wei
AU  - Wang W
FAU - Lin, Zhongqin
AU  - Lin Z
FAU - Chen, Qingyu
AU  - Chen Q
FAU - Zhang, Wei
AU  - Zhang W
FAU - Kou, Dongquan
AU  - Kou D
FAU - Shen, Yue
AU  - Shen Y
FAU - Cheng, Xiaojie
AU  - Cheng X
FAU - Rompis, Ferdinand An
AU  - Rompis FA
FAU - Peng, Lei
AU  - Peng L
FAU - Zhu Lu, Chuan
AU  - Zhu Lu C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110531
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Boric Acids)
RN  - 0 (Boron Compounds)
RN  - 0 (Collagen Type I)
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Messenger)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - R57ZHV85D4 (boric acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Bone Marrow Cells/*drug effects
MH  - Bone Morphogenetic Protein 7/metabolism
MH  - Boric Acids/pharmacology
MH  - Boron Compounds/*pharmacology
MH  - Calcium/metabolism
MH  - Cell Count
MH  - Cell Differentiation/drug effects/genetics
MH  - Cell Proliferation/drug effects
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Collagen Type I/biosynthesis
MH  - DNA Primers
MH  - Dose-Response Relationship, Drug
MH  - Genetic Markers
MH  - Humans
MH  - Osteocalcin/biosynthesis
MH  - Osteogenesis/*drug effects/genetics
MH  - Phenotype
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Stromal Cells/*drug effects
EDAT- 2011/06/01 06:00
MHDA- 2012/04/28 06:00
CRDT- 2011/06/01 06:00
PHST- 2011/04/07 00:00 [received]
PHST- 2011/05/19 00:00 [accepted]
PHST- 2011/06/01 06:00 [entrez]
PHST- 2011/06/01 06:00 [pubmed]
PHST- 2012/04/28 06:00 [medline]
AID - 10.1007/s12011-011-9094-x [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2011 Dec;144(1-3):306-15. doi: 10.1007/s12011-011-9094-x. 
      Epub 2011 May 31.