PMID- 21626024
OWN - NLM
STAT- MEDLINE
DCOM- 20120123
LR  - 20221207
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 92
IP  - 3
DP  - 2011 Nov
TI  - Effects of cryoprotectants on viability of Lactobacillus reuteri CICC6226.
PG  - 609-16
LID - 10.1007/s00253-011-3269-4 [doi]
AB  - Freeze-drying is commonly used to preserve probiotics, but it could cause cell 
      damage and loss of viability. The cryoprotectants play an important role in the 
      conservation of viability during freeze-drying. In this study, we investigated 
      the survival rates of Lactobacillus reuteri CICC6226 in the presence of 
      cryoprotectants such as sucrose, trehalose, and reconstituted skim milk (RSM). In 
      addition, we determined the activities of hexokinase (HK), pyruvate kinase (PK), 
      lactate dehydrogenase (LDH), and ATPases immediately following the freeze-drying. 
      The results showed that the differences in HK and PK activities with and without 
      the cryoprotectants during freeze-drying were not significant, but cell viability 
      and activities of LDH and ATPase were significantly different (P<0.01) prior to 
      and after freeze-drying. Meanwhile, the results showed that the maintenance of 
      the membrane integrity and fluidity was improved in the presence of the 10% 
      trehalose or 10% RSM than other treatments during freeze-drying. These results 
      have provided direct biochemical and metabolic evidence of injured cell during 
      freeze-drying. Freeze-drying damaged membrane structure and function of cell and 
      inactivated enzymes (LDH and ATPases). The results imply that LDH and ATPases are 
      key markers and could be used to evaluate the effect of cryoprotectants on 
      viability and metabolic activities of L. reuteri CICC6226 during freeze-drying.
FAU - Li, Baokun
AU  - Li B
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi, Jiangsu, People's Republic of China.
FAU - Tian, Fengwei
AU  - Tian F
FAU - Liu, Xiaoming
AU  - Liu X
FAU - Zhao, Jianxin
AU  - Zhao J
FAU - Zhang, Hao
AU  - Zhang H
FAU - Chen, Wei
AU  - Chen W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110529
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Cryoprotective Agents)
RN  - 57-50-1 (Sucrose)
RN  - B8WCK70T7I (Trehalose)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Adenosine Triphosphatases/metabolism
MH  - Animals
MH  - Cell Membrane/physiology
MH  - Cryoprotective Agents/*metabolism
MH  - Freeze Drying/*methods
MH  - Hexokinase/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Limosilactobacillus reuteri/*drug effects/enzymology/physiology/*radiation 
      effects
MH  - Membrane Fluidity/drug effects/radiation effects
MH  - Microbial Viability/*drug effects/*radiation effects
MH  - Milk
MH  - Pyruvate Kinase/metabolism
MH  - Sucrose/metabolism
MH  - Trehalose/metabolism
EDAT- 2011/06/01 06:00
MHDA- 2012/01/24 06:00
CRDT- 2011/06/01 06:00
PHST- 2011/01/07 00:00 [received]
PHST- 2011/03/23 00:00 [accepted]
PHST- 2011/03/22 00:00 [revised]
PHST- 2011/06/01 06:00 [entrez]
PHST- 2011/06/01 06:00 [pubmed]
PHST- 2012/01/24 06:00 [medline]
AID - 10.1007/s00253-011-3269-4 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2011 Nov;92(3):609-16. doi: 10.1007/s00253-011-3269-4. 
      Epub 2011 May 29.