PMID- 2162890
OWN - NLM
STAT- MEDLINE
DCOM- 19900727
LR  - 20141120
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 145
IP  - 1
DP  - 1990 Jul 1
TI  - Identification of high affinity receptors for human monocyte chemoattractant 
      protein-1 on human monocytes.
PG  - 292-7
AB  - The binding of human monocyte chemoattractant protein-1 (MCP-1) to human 
      monocytes was studied. MCP-1 was radioiodinated with Iodo-beads (Pierce Chemical 
      Co., Rockford, IL) without significant loss of biologic activity. 125I-MCP-1 
      binding to PBMC occurred within 5 min at 0 degrees C and the binding was 
      inhibited by unlabeled MCP-1 dose dependently but not by neutrophil 
      attractant/activation protein-1 or FMLP. 125I-MCP-1 bound to monocytes; no 
      significant binding to either neutrophils or lymphocytes was observed. Scatchard 
      plot analysis indicated that monocytes had a minimum of 1700 +/- 600 binding 
      sites per cell with a Kd of 1.9 +/- 0.2 x 10(-9) M. For analysis of binding by 
      flow cytometry, MCP-1 was biotinylated. In contrast to radioiodination, 
      biotinylation resulted in loss of activity; potency was 10-fold less, but the 
      efficacy was retained. Detection by flow cytometry of bound biotinylated MCP-1 
      with avidin-FITC confirmed results obtained with 125I-MCP-1. Biotinylated MCP-1 
      bound to monocytes but not to lymphocytes; and the binding was inhibited by a 
      100-fold excess of unlabeled MCP-1.
FAU - Yoshimura, T
AU  - Yoshimura T
AD  - Immunopathology Section, National Cancer Institute-Frederick Cancer Research 
      Facility, MD 21701.
FAU - Leonard, E J
AU  - Leonard EJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*physiology
MH  - *Chemotaxis, Leukocyte
MH  - Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - Lymphocytes/metabolism
MH  - Molecular Weight
MH  - Monocytes/*physiology
MH  - Receptors, Cell Surface/*metabolism
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1990 Jul 1;145(1):292-7.