PMID- 21630329
OWN - NLM
STAT- MEDLINE
DCOM- 20111209
LR  - 20211020
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 29
IP  - 12
DP  - 2011 Dec
TI  - Characterization of a cartilage-like engineered biomass using a self-aggregating 
      suspension culture model: molecular composition using FT-IRIS.
PG  - 1881-7
LID - 10.1002/jor.21467 [doi]
AB  - Maintenance of chondrocyte phenotype and robust expression and organization of 
      macromolecular components with suitable cartilaginous properties is an ultimate 
      goal in cartilage tissue engineering. We used a self-aggregating suspension 
      culture (SASC) method to produce an engineered cartilage, "cartilage tissue 
      analog" (CTA). With an objective of understanding the stability of phenotype of 
      the CTA over long periods, we cultured chondrocytes up to 4 years and analyzed 
      the matrix. Both early (eCTAs) (6 months) and aged (aCTAs) (4 years) showed type 
      II collagen throughout with higher concentrations near the edge. Using Fourier 
      transform-infrared imaging spectroscopy (FT-IRIS), proteoglycan/collagen ratio of 
      eCTA was 2.8 times greater than native cartilage at 1 week, but the ratio was 
      balanced to native level (p = 0.017) by 36 weeks. Surprisingly, aCTAs maintained 
      the hyaline characteristics, but there was evidence of calcification within the 
      tissue with a distinct range of intensities. Mineral/matrix ratio of those aCTA 
      with "intensive" calcification was significantly higher (p = 0.017) than the 
      "partial," but when compared to native bone the ratio of "intensive" aCTAs was 
      2.4 times lower. In this study we utilized the imaging approach of FT-IRIS and 
      have shown that a biomaterial formed is compositionally closely related to 
      natural cartilage for long periods in culture. We show that this culture platform 
      can maintain a CTA for extended periods of time (4 years) and under those 
      conditions signs of mineralization can be found. This method of cartilage tissue 
      engineering is a promising method to generate cartilaginous biomaterial and may 
      have potential to be utilized in both cartilage and boney repairs.
CI  - Copyright (c) 2011 Orthopaedic Research Society.
FAU - Kim, Minwook
AU  - Kim M
AD  - Department of Biological Sciences, University of Delaware, Newark, Delaware, USA.
FAU - Kraft, Jeffrey J
AU  - Kraft JJ
FAU - Volk, Andrew C
AU  - Volk AC
FAU - Pugarelli, Joan
AU  - Pugarelli J
FAU - Pleshko, Nancy
AU  - Pleshko N
FAU - Dodge, George R
AU  - Dodge GR
LA  - eng
GR  - P30 AR046121-06A1/AR/NIAMS NIH HHS/United States
GR  - R01 AR056145/AR/NIAMS NIH HHS/United States
GR  - P30 AR046121-05/AR/NIAMS NIH HHS/United States
GR  - AR046121/AR/NIAMS NIH HHS/United States
GR  - P30 AR046121/AR/NIAMS NIH HHS/United States
GR  - NIHARO56145/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110531
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Collagen Type II)
RN  - 0 (Proteoglycans)
SB  - IM
MH  - Animals
MH  - Bone Density
MH  - Cartilage, Articular/*cytology/metabolism
MH  - Cell Count
MH  - Cell Culture Techniques/*methods
MH  - Chondrocytes/*cytology/metabolism
MH  - Collagen Type II/metabolism
MH  - Femur
MH  - Hyalin/metabolism
MH  - Proteoglycans/metabolism
MH  - Spectroscopy, Fourier Transform Infrared/*methods
MH  - Sus scrofa
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
PMC - PMC4617763
MID - NIHMS295432
EDAT- 2011/06/02 06:00
MHDA- 2011/12/14 06:00
PMCR- 2015/10/23
CRDT- 2011/06/02 06:00
PHST- 2010/08/19 00:00 [received]
PHST- 2011/05/04 00:00 [accepted]
PHST- 2011/06/02 06:00 [entrez]
PHST- 2011/06/02 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
PHST- 2015/10/23 00:00 [pmc-release]
AID - 10.1002/jor.21467 [doi]
PST - ppublish
SO  - J Orthop Res. 2011 Dec;29(12):1881-7. doi: 10.1002/jor.21467. Epub 2011 May 31.