PMID- 21630487
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20130416
IS  - 1520-6033 (Electronic)
IS  - 1520-6033 (Linking)
VI  - 27
IP  - 4
DP  - 2011 Jul
TI  - Gas-permeable membranes and co-culture with fibroblasts enable high-density 
      hepatocyte culture as multilayered liver tissues.
PG  - 1146-53
LID - 10.1002/btpr.626 [doi]
AB  - To engineer reliable in vitro liver tissue equivalents expressing differentiated 
      hepatic functions at a high level and over a long period of time, it appears 
      necessary to have liver cells organized into a three-dimensional (3D) 
      multicellular structure closely resembling in vivo liver cytoarchitecture and 
      promoting both homotypic and heterotypic cell-cell contacts. In addition, such 
      high density 3D hepatocyte cultures should be adequately supplied with nutrients 
      and particularly with oxygen since it is one of the most limiting nutrients in 
      hepatocyte cultures. Here we propose a novel but simple hepatocyte culture system 
      in a microplate-based format, enabling high density hepatocyte culture as a 
      stable 3D-multilayer. Multilayered co-cultures of hepatocytes and 3T3 fibroblasts 
      were engineered on collagen-conjugated thin polydimethylsiloxane (PDMS) membranes 
      which were assembled on bottomless frames to enable oxygen diffusion through the 
      membrane. To achieve high density multilayered co-cultures, primary rat 
      hepatocytes were seeded in large excess what was rendered possible due to the 
      removal of oxygen shortage generally encountered in microplate-based hepatocyte 
      cultures. Hepatocyte/3T3 fibroblasts multilayered co-cultures were maintained for 
      at least 1 week; the so-cultured cells were normoxic and sustained differentiated 
      metabolic functions like albumin and urea synthesis at higher levels than 
      hepatocytes monocultures. Such a microplate-based cell culture system appears 
      suitable for engineering in vitro miniature liver tissues for implantation, 
      bioartificial liver (BAL) development, or chemical/drug screening.
CI  - Copyright (c) 2011 American Institute of Chemical Engineers (AIChE).
FAU - Evenou, Fanny
AU  - Evenou F
AD  - Institute of Industrial Science (IIS), University of Tokyo, Tokyo, Japan. 
      fanny.evenou@univ-paris-diderot.fr
FAU - Hamon, Morgan
AU  - Hamon M
FAU - Fujii, Teruo
AU  - Fujii T
FAU - Takeuchi, Shoji
AU  - Takeuchi S
FAU - Sakai, Yasuyuki
AU  - Sakai Y
LA  - eng
PT  - Journal Article
DEP - 20110531
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
RN  - 0 (Membranes, Artificial)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Cells, Cultured
MH  - Fibroblasts/*cytology
MH  - Hepatocytes/*cytology
MH  - Liver/*cytology
MH  - Liver, Artificial
MH  - Male
MH  - *Membranes, Artificial
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Engineering/methods
EDAT- 2011/06/02 06:00
MHDA- 2013/10/23 06:00
CRDT- 2011/06/02 06:00
PHST- 2010/11/23 00:00 [received]
PHST- 2011/03/18 00:00 [revised]
PHST- 2011/06/02 06:00 [entrez]
PHST- 2011/06/02 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
AID - 10.1002/btpr.626 [doi]
PST - ppublish
SO  - Biotechnol Prog. 2011 Jul;27(4):1146-53. doi: 10.1002/btpr.626. Epub 2011 May 31.