PMID- 21631380
OWN - NLM
STAT- MEDLINE
DCOM- 20111207
LR  - 20211020
IS  - 1557-8577 (Electronic)
IS  - 1549-1684 (Print)
IS  - 1549-1684 (Linking)
VI  - 14
IP  - 3
DP  - 2011 Jun
TI  - An exploratory analysis of the effects of a weight loss plus exercise program on 
      cellular quality control mechanisms in older overweight women.
PG  - 315-24
LID - 10.1089/rej.2010.1132 [doi]
AB  - Obese older adults are particularly susceptible to sarcopenia and have a higher 
      prevalence of disability than their peers of normal weight. Interventions to 
      improve body composition in late life are crucial to maintaining independence. 
      The main mechanisms underlying sarcopenia have not been determined conclusively, 
      but chronic inflammation, apoptosis, and impaired mitochondrial function are 
      believed to play important roles. It has yet to be determined whether impaired 
      cellular quality control mechanisms contribute to this process. The objective of 
      this study was to assess the effects of a 6-month weight loss program combined 
      with moderate-intensity exercise on the cellular quality control mechanisms 
      autophagy and ubiquitin-proteasome, as well as on inflammation, apoptosis, and 
      mitochondrial function, in the skeletal muscle of older obese women. The 
      intervention resulted in significant weight loss (8.0 +/- 3.9 % vs. 0.4 +/- 3.1% of 
      baseline weight, p = 0.002) and improvements in walking speed (reduced time to 
      walk 400 meters, - 20.4 +/- 16% vs. - 2.5 +/- 12%, p = 0.03). In the intervention 
      group, we observed a three-fold increase in messenger RNA (mRNA) levels of the 
      autophagy regulators LC3B, Atg7, and lysosome-associated membrane protein-2 
      (LAMP-2) compared to controls. Changes in mRNA levels of FoxO3A and its targets 
      MuRF1, MAFBx, and BNIP3 were on average seven-fold higher in the intervention 
      group compared to controls, but these differences were not statistically 
      significant. Tumor necrosis factor-alpha (TNF-alpha) mRNA levels were elevated after the 
      intervention, but we did not detect significant changes in the downstream 
      apoptosis markers caspase 8 and 3. Mitochondrial biogenesis markers (PGC1alpha and 
      TFAm) were increased by the intervention, but this was not accompanied by 
      significant changes in mitochondrial complex content and activity. In conclusion, 
      although exploratory in nature, this study is among the first to report the 
      stimulation of cellular quality control mechanisms elicited by a weight loss and 
      exercise program in older obese women.
FAU - Wohlgemuth, Stephanie E
AU  - Wohlgemuth SE
AD  - Department of Aging and Geriatrics, Institute on Aging, College of Medicine, 
      University of Florida, Gainesville, FL 32611-0910, USA. steffiw@ufl.edu
FAU - Lees, Hazel A
AU  - Lees HA
FAU - Marzetti, Emanuele
AU  - Marzetti E
FAU - Manini, Todd M
AU  - Manini TM
FAU - Aranda, Juan M
AU  - Aranda JM
FAU - Daniels, Michael J
AU  - Daniels MJ
FAU - Pahor, Marco
AU  - Pahor M
FAU - Perri, Michael G
AU  - Perri MG
FAU - Leeuwenburgh, Christian
AU  - Leeuwenburgh C
FAU - Anton, Stephen D
AU  - Anton SD
LA  - eng
GR  - K23 AT004251/AT/NCCIH NIH HHS/United States
GR  - P30 AG028740/AG/NIA NIH HHS/United States
GR  - P30AG028740/AG/NIA NIH HHS/United States
GR  - K23 AT004251-01A2/AT/NCCIH NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110601
PL  - United States
TA  - Rejuvenation Res
JT  - Rejuvenation research
JID - 101213381
SB  - IM
MH  - Aged
MH  - Apoptosis/genetics
MH  - *Autophagy/genetics
MH  - *Exercise
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Inflammation/complications/genetics
MH  - Middle Aged
MH  - Mitochondria/metabolism
MH  - Overweight/complications/genetics/*pathology/*therapy
MH  - *Weight Loss/genetics
PMC - PMC3136739
EDAT- 2011/06/03 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/06/01
CRDT- 2011/06/03 06:00
PHST- 2011/06/03 06:00 [entrez]
PHST- 2011/06/03 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/06/01 00:00 [pmc-release]
AID - 10.1089/rej.2010.1132 [pii]
AID - 10.1089/rej.2010.1132 [doi]
PST - ppublish
SO  - Rejuvenation Res. 2011 Jun;14(3):315-24. doi: 10.1089/rej.2010.1132. Epub 2011 
      Jun 1.