PMID- 21633184
OWN - NLM
STAT- MEDLINE
DCOM- 20111212
LR  - 20211020
IS  - 2092-6413 (Electronic)
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 43
IP  - 7
DP  - 2011 Jul 30
TI  - Inhibitory effect of (-)-epigallocatechin gallate on titanium particle-induced 
      TNF-alpha release and in vivo osteolysis.
PG  - 411-8
LID - 10.3858/emm.2011.43.7.045 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) and inflammatory cytokines released from 
      activated macrophages in response to particulate debris greatly impact 
      periprosthetic bone loss and consequent implant failure. In the present study, we 
      found that a major polyphenolic component of green tea, (-)-epigallocatechin 
      gallate (EGCG), inhibited Ti particle-induced TNF-alpha release in macrophages in 
      vitro and calvarial osteolysis in vivo. The Ti stimulation of macrophages 
      released TNF-alpha in a dose- and time-dependent manner, and EGCG substantially 
      suppressed Ti particle-induced TNF-alpha release. Analysis of signaling pathway 
      showed that EGCG inhibited the Ti-induced c-Jun N-terminus kinase (JNK) 
      activation and inhibitory kappaB (IkappaB) degradation, and consequently the Ti-induced 
      transcriptional activation of AP-1 and NF-kappaB. In a mouse calvarial osteolysis 
      model, EGCG inhibited Ti particle-induced osteolysis in vivo by suppressing TNF-a 
      expression and osteoclast formation. Therefore, EGCG may be a potential candidate 
      compound for osteolysis prevention and treatment as well as aseptic loosening 
      after total replacement arthroplasty.
FAU - Jin, Shan
AU  - Jin S
AD  - Skeletal Diseases Genome Research Center Kyungpook National University Hospital.
FAU - Park, Ju Young
AU  - Park JY
FAU - Hong, Jung Min
AU  - Hong JM
FAU - Kim, Tae Ho
AU  - Kim TH
FAU - Shin, Hong In
AU  - Shin HI
FAU - Park, Eui Kyun
AU  - Park EK
FAU - Kim, Shin Yoon
AU  - Kim SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (NF-kappa B)
RN  - 0 (Particulate Matter)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - D1JT611TNE (Titanium)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
SB  - IM
MH  - Animals
MH  - Catechin/*analogs & derivatives/pharmacology
MH  - Cell Line
MH  - Implants, Experimental
MH  - Macrophages/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinase 8/metabolism
MH  - NF-kappa B/metabolism
MH  - Osteolysis/chemically induced/*metabolism/prevention & control
MH  - Particulate Matter/*adverse effects
MH  - Prosthesis Failure
MH  - Signal Transduction/drug effects
MH  - Skull/*drug effects/pathology
MH  - Titanium/*adverse effects
MH  - Transcription Factor AP-1/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
PMC - PMC3158500
EDAT- 2011/06/03 06:00
MHDA- 2011/12/14 06:00
PMCR- 2011/07/31
CRDT- 2011/06/03 06:00
PHST- 2011/06/03 06:00 [entrez]
PHST- 2011/06/03 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
PHST- 2011/07/31 00:00 [pmc-release]
AID - emm.2010.43.045 [pii]
AID - 10.3858/emm.2011.43.7.045 [doi]
PST - ppublish
SO  - Exp Mol Med. 2011 Jul 30;43(7):411-8. doi: 10.3858/emm.2011.43.7.045.