PMID- 21637390
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Print)
IS  - 1598-2629 (Linking)
VI  - 11
IP  - 2
DP  - 2011 Apr
TI  - Mycobacterial Heparin-binding Hemagglutinin Antigen Activates Inflammatory 
      Responses through PI3-K/Akt, NF-kappaB, and MAPK Pathways.
PG  - 123-33
LID - 10.4110/in.2011.11.2.123 [doi]
AB  - BACKGROUND: Mycobacterium tuberculosis (Mtb) heparin binding hemagglutinin (HBHA) 
      is an Ag known to evoke effective host immune responses during tuberculosis 
      infection. However, the molecular basis of the host immune response to HBHA has 
      not been fully characterized. In this study, we examined the molecular mechanisms 
      by which HBHA can induce the expression of proinflammatory cytokines in 
      macrophages. METHODS: HBHA-induced mRNA and protein levels of proinflammatory 
      cytokines were determined in bone marrow-derived macrophages (BMDMs) using RT-PCR 
      and ELISA analysis. The roles of intracellular signaling pathways for NF-kappaB, 
      PI3-K/Akt, and MAPKs were investigated in macrophage proinflammatory responses 
      after stimulation with HBHA. RESULTS: HBHA robustly activated the expression of 
      mRNA and protein of both TNF-alpha and IL-6, and induced phosphorylation of NF-kappaB, 
      Akt, and MAPKs in BMDMs. Both TNF-alpha and IL-6 production by HBHA was regulated by 
      the NF-kappaB, PI3-K, and MAPK pathways. Furthermore, PI3-K activity was required for 
      the HBHA-induced activation of ERK1/2 and p38 MAPK, but not JNK, pathways. 
      CONCLUSION: These data suggest that mycobacterial HBHA significantly induces 
      proinflammatory responses through crosstalk between the PI3-K and MAPK pathways 
      in macrophages.
FAU - Kim, Ki-Hye
AU  - Kim KH
AD  - Department of Microbiology, College of Medicine, Chungnam National University, 
      Daejeon 301-747, Korea.
FAU - Yang, Chul-Su
AU  - Yang CS
FAU - Shin, A-Rum
AU  - Shin AR
FAU - Jeon, So-Ra
AU  - Jeon SR
FAU - Park, Jeong-Kyu
AU  - Park JK
FAU - Kim, Hwa-Jung
AU  - Kim HJ
FAU - Jo, Eun-Kyeong
AU  - Jo EK
LA  - eng
PT  - Journal Article
DEP - 20110430
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC3100523
OTO - NOTNLM
OT  - Heparin-binding hemagglutinin (HBHA)
OT  - MAPK
OT  - Macrophages
OT  - Mycobacterium tuberculosis
OT  - NF-kappaB
OT  - PI3-K
COIS- The authors have no financial conflict of interest.
EDAT- 2011/06/04 06:00
MHDA- 2011/06/04 06:01
PMCR- 2011/04/01
CRDT- 2011/06/04 06:00
PHST- 2011/03/18 00:00 [received]
PHST- 2011/04/04 00:00 [revised]
PHST- 2011/04/14 00:00 [accepted]
PHST- 2011/06/04 06:00 [entrez]
PHST- 2011/06/04 06:00 [pubmed]
PHST- 2011/06/04 06:01 [medline]
PHST- 2011/04/01 00:00 [pmc-release]
AID - 10.4110/in.2011.11.2.123 [doi]
PST - ppublish
SO  - Immune Netw. 2011 Apr;11(2):123-33. doi: 10.4110/in.2011.11.2.123. Epub 2011 Apr 
      30.