PMID- 21637581 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20121004 LR - 20220309 IS - 1678-4685 (Electronic) IS - 1415-4757 (Print) IS - 1415-4757 (Linking) VI - 33 IP - 4 DP - 2010 Oct TI - Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut. PG - 714-8 LID - 10.1590/S1415-47572010005000079 [doi] AB - The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small "A chromosomes" characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be considered as a B or non-A genome species. The pattern of heterochromatic bands and rDNA loci of A. trinitensis differ markedly from any of the complements of A. hypogaea, suggesting that the former species is unlikely to be one of the wild diploid progenitors of the latter. FAU - Robledo, German AU - Robledo G AD - Instituto de Botanica del Nordeste, Corrientes Argentina. FAU - Lavia, Graciela I AU - Lavia GI FAU - Seijo, Guillermo AU - Seijo G LA - eng PT - Journal Article DEP - 20101201 PL - Brazil TA - Genet Mol Biol JT - Genetics and molecular biology JID - 100883590 PMC - PMC3036135 OTO - NOTNLM OT - CMA/DAPI bands OT - genome donors OT - peanut genetic origin OT - rDNA loci EDAT- 2011/06/04 06:00 MHDA- 2011/06/04 06:01 PMCR- 2010/10/01 CRDT- 2011/06/04 06:00 PHST- 2009/10/20 00:00 [received] PHST- 2010/05/20 00:00 [accepted] PHST- 2011/06/04 06:00 [entrez] PHST- 2011/06/04 06:00 [pubmed] PHST- 2011/06/04 06:01 [medline] PHST- 2010/10/01 00:00 [pmc-release] AID - 10.1590/S1415-47572010005000079 [doi] PST - ppublish SO - Genet Mol Biol. 2010 Oct;33(4):714-8. doi: 10.1590/S1415-47572010005000079. Epub 2010 Dec 1.