PMID- 21637602
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20230527
IS  - 1678-4685 (Electronic)
IS  - 1415-4757 (Print)
IS  - 1415-4757 (Linking)
VI  - 33
IP  - 1
DP  - 2010 Jan
TI  - Chromosomal localization and molecular marker development of the 
      lipopolysaccharide and beta-1,3-glucan binding protein gene in the Zhikong 
      scallop Chlamys farreri (Jones et Preston) (Pectinoida, Pectinidae).
PG  - 36-43
LID - 10.1590/S1415-47572010005000015 [doi]
AB  - Zhikong scallop Chlamys farreri (Jones et Preston) is an economically important 
      species in China. Understanding its immune system would be of great help in 
      controlling diseases. In the present study, an important immunity-related gene, 
      the Lipopolysaccharide and Beta-1,3-glucan Binding Protein (LGBP) gene, was 
      located on C. farreri chromosomes by mapping several lgbp-containing BAC clones 
      through fluorescence in situ hybridization (FISH). Through the localization of 
      various BAC clones, it was shown that only one locus of this gene existed in the 
      genome of C. farreri, and that this was located on the long arm of a pair of 
      homologous chromosomes. Molecular markers, consisting of eight single nucleotide 
      polymorphism (SNPs) markers and one insertion-deletion (indel), were developed 
      from the LGBP gene. Indel marker testing in an F1 family revealed slightly 
      distorted segregation (p = 0.0472). These markers can be used to map the LGBP 
      gene to the linkage map and assign the linkage group to the corresponding 
      chromosome. Segregation distortion of the indel marker indicated genes with 
      deleterious alleles might exist in the surrounding region of the LGBP gene.
FAU - Huan, Pin
AU  - Huan P
AD  - Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese 
      Academy of Sciences, Qingdao P.R. China.
FAU - Zhang, Xiaojun
AU  - Zhang X
FAU - Li, Fuhua
AU  - Li F
FAU - Zhang, Yang
AU  - Zhang Y
FAU - Zhao, Cui
AU  - Zhao C
FAU - Xiang, Jianhai
AU  - Xiang J
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
DEP - 20100301
PL  - Brazil
TA  - Genet Mol Biol
JT  - Genetics and molecular biology
JID - 100883590
PMC - PMC3036087
OTO - NOTNLM
OT  - Chlamys farreri
OT  - LGBP
OT  - SNP
OT  - fluorescence in situ hybridization (FISH)
OT  - indel
EDAT- 2010/01/01 00:00
MHDA- 2010/01/01 00:01
PMCR- 2010/01/01
CRDT- 2011/06/04 06:00
PHST- 2009/03/03 00:00 [received]
PHST- 2009/08/17 00:00 [accepted]
PHST- 2011/06/04 06:00 [entrez]
PHST- 2010/01/01 00:00 [pubmed]
PHST- 2010/01/01 00:01 [medline]
PHST- 2010/01/01 00:00 [pmc-release]
AID - 10.1590/S1415-47572010005000015 [doi]
PST - ppublish
SO  - Genet Mol Biol. 2010 Jan;33(1):36-43. doi: 10.1590/S1415-47572010005000015. Epub 
      2010 Mar 1.