PMID- 21637955
OWN - NLM
STAT- MEDLINE
DCOM- 20120501
LR  - 20211020
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 2
DP  - 2012 Feb
TI  - Aldose reductase regulates TNF-alpha-induced inducible nitric oxide synthase 
      expression in human mesangial cells.
PG  - 1815-22
LID - 10.1007/s11033-011-0923-y [doi]
AB  - Glomerulonephritis is one of the most important causes of renal failure, which is 
      accompanied with production of Nitric Oxide (NO) synthesized by inducible nitric 
      oxide synthase (iNOS). Aldose reductase (AR) is the key enzyme in polyol pathway 
      and plays an important role in glucose metabolism. Here, we report our finding 
      that AR regulates tumor-necrosis-factor-alpha-induced (TNF-alpha-induced) iNOS expression 
      in human mesangial cells (HMC) via nuclear factor kappaB (NFkappaB) signal pathway. The 
      TNF-alpha-induced iNOS expression in HMC with different level of AR was measured by 
      Real-time PCR and Western blot. The activation of signal pathway was analyzed by 
      Western blot and electrophoretic mobility shift assay (EMSA). In cultured HMC, 
      TNF-alpha induces the expression of iNOS, which is attenuated by knockdown AR with 
      siRNA. On the other side, transfection with pcDNA3.1-AR gets the consistent 
      conclusion. Furthermore, knockdown of AR attenuated activity of NFkappaB, suggesting 
      that the effects of AR on this pathway may result in the reduced iNOS 
      transcription and expression. Taken together, these data demonstrate the role of 
      AR in regulating iNOS expression induced by TNF-alpha in cultured HMC, indicating the 
      novel function of AR in glomerulonephritis besides glucose metabolism.
FAU - Zhao, Jingjing
AU  - Zhao J
AD  - Department of Pathology, Shanghai Medical School, Fudan University, 138 Yi Xue 
      Yuan Road, Shanghai 200032, China. 072101049@fudan.edu.cn
FAU - Jiang, Tao
AU  - Jiang T
FAU - Li, Hui
AU  - Li H
FAU - Zhang, Yuejuan
AU  - Zhang Y
FAU - Zhang, Nong
AU  - Zhang N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110603
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.1.1.21 (Aldehyde Reductase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Aldehyde Reductase/*metabolism
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Electrophoretic Mobility Shift Assay
MH  - Gene Expression Regulation, Enzymologic/genetics/*physiology
MH  - Glomerulonephritis/*metabolism
MH  - Humans
MH  - Mesangial Cells/*enzymology
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide Synthase Type II/*metabolism
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2011/06/04 06:00
MHDA- 2012/05/02 06:00
CRDT- 2011/06/04 06:00
PHST- 2010/03/14 00:00 [received]
PHST- 2011/05/21 00:00 [accepted]
PHST- 2011/06/04 06:00 [entrez]
PHST- 2011/06/04 06:00 [pubmed]
PHST- 2012/05/02 06:00 [medline]
AID - 10.1007/s11033-011-0923-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Feb;39(2):1815-22. doi: 10.1007/s11033-011-0923-y. Epub 2011 
      Jun 3.