PMID- 21639146
OWN - NLM
STAT- MEDLINE
DCOM- 20110909
LR  - 20211020
IS  - 1520-4995 (Electronic)
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 50
IP  - 26
DP  - 2011 Jul 5
TI  - Oxidation and loss of heme in soluble guanylyl cyclase from Manduca sexta.
PG  - 5813-5
LID - 10.1021/bi200794c [doi]
AB  - Oxidation and loss of heme in soluble guanylyl/guanylate cyclase (sGC), the 
      nitric oxide receptor, is thought to be a major contributor to cardiovascular 
      disease and is the target of compounds BAY 58-2667 and HMR1766. Using 
      spectroelectrochemical titration, we found a truncated sGC to be highly stable in 
      the ferrous state (234 mV) and to bind ferrous heme tightly even in the presence 
      of NO, despite the NO-induced release of the proximal histidine. In contrast, 
      oxidized sGC readily loses ferric heme to myoglobin (0.47 +/- 0.02 h(-1)). 
      Peroxynitrite, the presumed cellular oxidant, readily oxidizes sGC in 5 mM 
      glutathione.
FAU - Fritz, Bradley G
AU  - Fritz BG
AD  - Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 
      85721, United States.
FAU - Hu, Xiaohui
AU  - Hu X
FAU - Brailey, Jacqueline L
AU  - Brailey JL
FAU - Berry, Robert E
AU  - Berry RE
FAU - Walker, F Ann
AU  - Walker FA
FAU - Montfort, William R
AU  - Montfort WR
LA  - eng
GR  - R01 HL054826/HL/NHLBI NIH HHS/United States
GR  - T32 GM008804/GM/NIGMS NIH HHS/United States
GR  - R01 HL054826-14/HL/NHLBI NIH HHS/United States
GR  - HL054826/HL/NHLBI NIH HHS/United States
GR  - R01 GM077390/GM/NIGMS NIH HHS/United States
GR  - R01 HL062969/HL/NHLBI NIH HHS/United States
GR  - HL062969/HL/NHLBI NIH HHS/United States
GR  - R01 HL062969-11/HL/NHLBI NIH HHS/United States
GR  - R01 GM077390-04/GM/NIGMS NIH HHS/United States
GR  - GM077390/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110610
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 42VZT0U6YR (Heme)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - EC 4.6.1.2 (Soluble Guanylyl Cyclase)
SB  - IM
MH  - Animals
MH  - Guanylate Cyclase/*chemistry/*metabolism
MH  - Heme/*metabolism
MH  - Kinetics
MH  - Manduca/*enzymology
MH  - Oxidation-Reduction
MH  - Receptors, Cytoplasmic and Nuclear/*chemistry/*metabolism
MH  - Soluble Guanylyl Cyclase
PMC - PMC3128461
MID - NIHMS302625
EDAT- 2011/06/07 06:00
MHDA- 2011/09/10 06:00
PMCR- 2012/07/05
CRDT- 2011/06/07 06:00
PHST- 2011/06/07 06:00 [entrez]
PHST- 2011/06/07 06:00 [pubmed]
PHST- 2011/09/10 06:00 [medline]
PHST- 2012/07/05 00:00 [pmc-release]
AID - 10.1021/bi200794c [doi]
PST - ppublish
SO  - Biochemistry. 2011 Jul 5;50(26):5813-5. doi: 10.1021/bi200794c. Epub 2011 Jun 10.