PMID- 21640524
OWN - NLM
STAT- MEDLINE
DCOM- 20120531
LR  - 20181201
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 152
IP  - 3-4
DP  - 2011 Sep 28
TI  - The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for 
      anterograde axonal transport.
PG  - 270-9
LID - 10.1016/j.vetmic.2011.05.012 [doi]
AB  - In this study, we examined the functional role of bovine herpesvirus type 1 
      (BHV-1) Us9 acidic domain residues 83-90 in the anterograde axonal transport of 
      the virus in calves (natural host), rabbits, and in cultured neurons. A mutant 
      virus strain lacking Us9 residues 83-90 (BHV-1 Us9 Delta83-90) and the rescued virus 
      (BHV-1 Us9 R83-90) replicated efficiently in the nasal and ocular epithelium 
      during primary infection and established latency in the trigeminal ganglia (TG). 
      However, upon reactivation from latency, only the BHV-1 Us9 R83-90 virus was 
      detected in nasal and ocular swabs of animals. In compartmentalized, rabbit 
      primary dorsal root ganglia (DRG) neuron cultures, the Us9-deleted BHV-1, BHV-1 
      Us9 Delta83-90 and BHV-1 Us9 R83-90 viruses were transported efficiently in the 
      retrograde direction. However, only the BHV-1 Us9 R83-90 virus was transported in 
      an anterograde direction. These studies suggested that the Us9 acidic domain 
      residues located between 83 and 90 were required for axonal anterograde 
      transport.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Chowdhury, S I
AU  - Chowdhury SI
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana 
      State University, Baton Rouge, LA 70803, USA. Chowdh@lsu.edu
FAU - Brum, M C S
AU  - Brum MC
FAU - Coats, C
AU  - Coats C
FAU - Doster, A
AU  - Doster A
FAU - Wei, Huiyong
AU  - Wei H
FAU - Jones, C
AU  - Jones C
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110513
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (US9 protein, bovine herpesvirus 1)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Axonal Transport
MH  - Cattle
MH  - Cell Line
MH  - Ganglia, Spinal/virology
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Herpesvirus 1, Bovine/*physiology
MH  - Molecular Sequence Data
MH  - Neurons/virology
MH  - Protein Structure, Tertiary
MH  - Rabbits
MH  - Trigeminal Ganglion/virology
MH  - Viral Envelope Proteins/*chemistry/*metabolism
EDAT- 2011/06/07 06:00
MHDA- 2012/06/01 06:00
CRDT- 2011/06/07 06:00
PHST- 2011/02/22 00:00 [received]
PHST- 2011/05/05 00:00 [revised]
PHST- 2011/05/06 00:00 [accepted]
PHST- 2011/06/07 06:00 [entrez]
PHST- 2011/06/07 06:00 [pubmed]
PHST- 2012/06/01 06:00 [medline]
AID - S0378-1135(11)00276-8 [pii]
AID - 10.1016/j.vetmic.2011.05.012 [doi]
PST - ppublish
SO  - Vet Microbiol. 2011 Sep 28;152(3-4):270-9. doi: 10.1016/j.vetmic.2011.05.012. 
      Epub 2011 May 13.