PMID- 21641124
OWN - NLM
STAT- MEDLINE
DCOM- 20120531
LR  - 20181201
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 152
IP  - 3-4
DP  - 2011 Sep 28
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV) influences infection 
      dynamics of porcine circovirus type 2 (PCV2) subtypes PCV2a and PCV2b by 
      prolonging PCV2 viremia and shedding.
PG  - 235-46
LID - 10.1016/j.vetmic.2011.05.005 [doi]
AB  - The objective of this study was to determine the effect of porcine reproductive 
      and respiratory syndrome virus (PRRSV) infection on porcine circovirus type 2 
      (PCV2) subtypes a (PCV2a) or b (PCV2b) viremia and shedding characteristics in 
      oral, nasal and fecal samples in experimentally infected pigs. Twenty-three, 2- 
      to 6-week-old pigs were randomly divided into five groups: negative control 
      (n=3), PCV2a-I (n=5), PCV2a-PRRSV-CoI (n=5), PCV2b-I (n=5), and PCV2b-PRRSV-CoI 
      (n=5). Blood, oral, nasal and fecal swabs were collected in regular intervals 
      from day post inoculation (dpi) 0 until dpi 70 and tested by quantitative 
      real-time PCR for the presence and amount of PCV2 DNA and by ELISA for the 
      presence of PCV2-specific antibodies. The results indicate that there were 
      significantly (P<0.05) higher loads of PCV2a and PCV2b DNA in serum, oral swabs, 
      nasal swabs and fecal swabs and a higher prevalence of detectable PCV2 antigen in 
      tissues of pigs concurrently infected with PCV2 and PRRSV compared to pigs 
      singularly infected with PCV2 further confirming that PRRSV enhances replication 
      of PCV2. Moreover, PRRSV infection significantly prolonged the presence of PCV2 
      DNA in serum and increased the amount of PCV2 DNA in oral and nasal secretions 
      and fecal excretions in the later stages of infection between dpi 28 and 70. 
      Shedding patterns were similar between groups infected with PCV2a and PCV2b, 
      indicating that there was no subtype-specific interaction with the PRRSV isolate 
      used in this study. The results from this study highlight the interaction between 
      PRRSV and PCV2 and the importance of controlling PRRSV infection in order to 
      reduce PCV2 virus loads in pig populations.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Sinha, A
AU  - Sinha A
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, 
      USA.
FAU - Shen, H G
AU  - Shen HG
FAU - Schalk, S
AU  - Schalk S
FAU - Beach, N M
AU  - Beach NM
FAU - Huang, Y W
AU  - Huang YW
FAU - Meng, X J
AU  - Meng XJ
FAU - Halbur, P G
AU  - Halbur PG
FAU - Opriessnig, T
AU  - Opriessnig T
LA  - eng
PT  - Journal Article
DEP - 20110511
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood/genetics
MH  - Circoviridae Infections/immunology/*veterinary/virology
MH  - *Circovirus
MH  - *Coinfection
MH  - Immunoglobulin G/blood
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Real-Time Polymerase Chain Reaction
MH  - Specific Pathogen-Free Organisms
MH  - Sus scrofa
MH  - Swine
MH  - Swine Diseases/immunology/*virology
MH  - Viremia
MH  - Virus Shedding
EDAT- 2011/06/07 06:00
MHDA- 2012/06/01 06:00
CRDT- 2011/06/07 06:00
PHST- 2010/12/02 00:00 [received]
PHST- 2011/03/19 00:00 [revised]
PHST- 2011/05/04 00:00 [accepted]
PHST- 2011/06/07 06:00 [entrez]
PHST- 2011/06/07 06:00 [pubmed]
PHST- 2012/06/01 06:00 [medline]
AID - S0378-1135(11)00269-0 [pii]
AID - 10.1016/j.vetmic.2011.05.005 [doi]
PST - ppublish
SO  - Vet Microbiol. 2011 Sep 28;152(3-4):235-46. doi: 10.1016/j.vetmic.2011.05.005. 
      Epub 2011 May 11.