PMID- 21642001
OWN - NLM
STAT- MEDLINE
DCOM- 20111024
LR  - 20110704
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 31
IP  - 2
DP  - 2011 Aug
TI  - Viral hemorrhagic septicaemia virus (VHSV) up-regulates the cytotoxic activity 
      and the perforin/granzyme pathway in the rainbow trout RTS11 cell line.
PG  - 252-9
LID - 10.1016/j.fsi.2011.05.010 [doi]
AB  - A survey of immune-relevant genes that might be up-regulated in response to viral 
      hemorrhagic septicaemia virus (VHSV) in the rainbow trout monocyte-macrophage 
      cell line, RTS11, unexpectedly revealed an increased expression of perforin (PRF) 
      and granzyme (GRZ) genes, which represent components of the major cytotoxic 
      pathway. The natural killer-enhancing factor (NKEF), also known to modulate 
      cytotoxic activity, was up-regulated at the gene but strikingly down-regulated at 
      protein level. The expression of these genes was not affected in head kidney 
      leukocytes (HKLs) infected with VHSV, leading us to evaluate the potential 
      cytotoxic activity of RTS11 and HKLs. For the first time, the cytotoxic activity 
      of RTS11 against xenogeneic targets has been demonstrated, although this was 
      modest relative to HKLs. Yet the activity in RTS11 was significantly increased by 
      VHSV, as in HKLs. This cytotoxic activity elicited by viral infection appeared to 
      require viral gene expression because inactivated VHSV failed to increase RTS11 
      cytotoxic activity. As for other immune functions, RTS11 cells provide a model 
      for further studying cytotoxic activities of fish monocyte-macrophages.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Ordas, M C
AU  - Ordas MC
AD  - Centro de Investigacion en Sanidad Animal (CISA-INIA), Carretera de Algete a El 
      Casar km. 8.1, Valdeolmos 28130, Madrid, Spain.
FAU - Cuesta, A
AU  - Cuesta A
FAU - Mercado, L
AU  - Mercado L
FAU - Bols, N C
AU  - Bols NC
FAU - Tafalla, C
AU  - Tafalla C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110527
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 126465-35-8 (Perforin)
RN  - EC 3.4.21.- (Granzymes)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Cytotoxicity, Immunologic
MH  - Fish Proteins/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Genes, Viral
MH  - Granzymes/genetics/*metabolism
MH  - Hemorrhagic Septicemia, Viral/*immunology/virology
MH  - Kidney/cytology
MH  - Leukocytes/immunology
MH  - Macrophages/immunology
MH  - Monocytes/immunology
MH  - *Novirhabdovirus
MH  - *Oncorhynchus mykiss
MH  - Perforin/genetics/*metabolism
MH  - Up-Regulation
EDAT- 2011/06/07 06:00
MHDA- 2011/10/25 06:00
CRDT- 2011/06/07 06:00
PHST- 2011/02/23 00:00 [received]
PHST- 2011/05/11 00:00 [revised]
PHST- 2011/05/11 00:00 [accepted]
PHST- 2011/06/07 06:00 [entrez]
PHST- 2011/06/07 06:00 [pubmed]
PHST- 2011/10/25 06:00 [medline]
AID - S1050-4648(11)00184-7 [pii]
AID - 10.1016/j.fsi.2011.05.010 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2011 Aug;31(2):252-9. doi: 10.1016/j.fsi.2011.05.010. 
      Epub 2011 May 27.