PMID- 21644226
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20110606
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 28
IP  - 3
DP  - 2011 Jun
TI  - [Identification of a novel allele HLA-B*15:129 by polymerase chain reaction with 
      allele group-specific primers].
PG  - 293-5
LID - 10.3760/cma.j.issn.1003-9406.2011.03.012 [doi]
AB  - OBJECTIVE: To analyze the sequence of the exons 2-4 of human leukocyte antigen 
      (HLA) novel allele HLA-B*15:129. METHODS: DNA of the proband was extracted from 
      whole blood by commercial DNA extraction kit. The amplification for HLA-B exons 
      2-4 was performed separately by polymerase chain reaction (PCR) with allele group 
      specific primers. The PCR products were digested with enzymes and then directly 
      sequenced for exons 2-4 of HLA-B locus in both directions. RESULTS: Sequencing 
      results showed the HLA-B alleles of the proband included B*07:02 and a novel 
      allele. The sequence of the novel allele has been submitted to GenBank (accession 
      no. EF473219) and the allele has been officially named B*15:129 by the WHO 
      Nomenclature Committee. Comparing with the HLA-B*15:01:01:01, the sequence of 
      exons 2-4 of HLA-B*15:129 showed three nucleotide difference in exon 3 at 
      positions 362 and 363 from GG to AT and positions 369 from C to T, which resulted 
      in an amino acid change from Arg to Asn at codon 97. CONCLUSION: A novel HLA-B 
      allele was identified and has been officially named B15:129 by the WHO 
      Nomenclature Committee.
FAU - Han, Zhe-dong
AU  - Han ZD
AD  - Blood Center of Zhejiang Province, Key Laboratory of Blood Safety of Ministry of 
      Health, Hangzhou, Zhejiang, 310006 P. R. China.
FAU - Wang, Wei
AU  - Wang W
FAU - Zhang, Wei
AU  - Zhang W
FAU - He, Jun-jun
AU  - He JJ
FAU - Chen, Nan-ying
AU  - Chen NY
FAU - Zhu, Fa-ming
AU  - Zhu FM
FAU - Lv, Hang-jun
AU  - Lv HJ
FAU - Yan, Li-xing
AU  - Yan LX
LA  - chi
SI  - GENBANK/EF473219
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA Primers)
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - DNA Primers
MH  - Exons
MH  - HLA-B Antigens/*genetics
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Molecular Typing
MH  - Polymerase Chain Reaction
EDAT- 2011/06/07 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/06/07 06:00
PHST- 2011/06/07 06:00 [entrez]
PHST- 2011/06/07 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 940628060 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2011.03.012 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Jun;28(3):293-5. doi: 
      10.3760/cma.j.issn.1003-9406.2011.03.012.