PMID- 21644228
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20110606
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 28
IP  - 3
DP  - 2011 Jun
TI  - [Cloning and sequencing analysis of a novel allele HLA-A*02:251].
PG  - 300-3
LID - 10.3760/cma.j.issn.1003-9406.2011.03.014 [doi]
AB  - OBJECTIVE: To identify a novel human leukocyte antigen (HLA) allele A*02:251 and 
      analyze the sequences in Chinese population. METHODS: Routine HLA-A, -B, -DRB1 
      high resolution genotyping for healthy Chinese donors and patients was performed 
      with polymerase chain reaction-sequence based typing. An unknown HLA-A allele was 
      initially detected by HLA typing in the healthy donor. Genomic DNA of the HLA-A 
      locus in the proband was amplified, the amplified product was cloned by PMD18-T 
      to split the two alleles, and selected clones were sequenced. RESULTS: The 
      sequencing results showed that a normal A*02:06:01 and a novel A*02:251 variant 
      allele were identified. The sequence of the novel allele has been submitted to 
      GenBank (HM245348). Nucleotide sequence alignments with HLA-A allele from the 
      IMGT/HLA Sequence Database showed that the novel A*02 variant allele differed 
      from the closest allele A*02:01:01:01 by nt 383 G to C (codon 128 GAG to GAC) in 
      exon 3, which resulted in one amino acid substitution of Glu to Asp. The HLA-A, 
      B, C and DQB1 alleles of the healthy donor did not match with that of the 
      patient. CONCLUSION: This novel allele is officially designated as HLA-A*02:251 
      by World Health Organization(WHO) Nomenclature Committee (Submission ID 
      HWS10010755). The sequence of HLA-A locus in exon 3 is confirmed to be 
      polymorphic in Chinese population.
FAU - Jin, Shi-zheng
AU  - Jin SZ
AD  - Shenzhen Blood Center Institute, Shenzhen HLA Laboratory of China Marrow Donor 
      Program, Shenzhen, Guangdong, 518035 P. R. China.
FAU - Gao, Su-qing
AU  - Gao SQ
FAU - Zou, Hong-yan
AU  - Zou HY
FAU - Deng, Zhi-hui
AU  - Deng ZH
LA  - chi
SI  - GENBANK/HM245348
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (HLA-A Antigens)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Exons/genetics
MH  - Genotype
MH  - HLA-A Antigens/*genetics
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2011/06/07 06:00
MHDA- 2011/10/01 06:00
CRDT- 2011/06/07 06:00
PHST- 2011/06/07 06:00 [entrez]
PHST- 2011/06/07 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 940628062 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2011.03.014 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Jun;28(3):300-3. doi: 
      10.3760/cma.j.issn.1003-9406.2011.03.014.