PMID- 21644679
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20220318
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 70
IP  - 23
DP  - 1998 Dec 1
TI  - Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).
PG  - 4974-84
LID - 10.1021/ac980656z [doi]
AB  - This paper describes a procedure that makes it possible to design and fabricate 
      (including sealing) microfluidic systems in an elastomeric material [Formula: see 
      text] poly(dimethylsiloxane) (PDMS) [Formula: see text] in less than 24 h. A 
      network of microfluidic channels (with width >20 mum) is designed in a CAD 
      program. This design is converted into a transparency by a high-resolution 
      printer; this transparency is used as a mask in photolithography to create a 
      master in positive relief photoresist. PDMS cast against the master yields a 
      polymeric replica containing a network of channels. The surface of this replica, 
      and that of a flat slab of PDMS, are oxidized in an oxygen plasma. These oxidized 
      surfaces seal tightly and irreversibly when brought into conformal contact. 
      Oxidized PDMS also seals irreversibly to other materials used in microfluidic 
      systems, such as glass, silicon, silicon oxide, and oxidized polystyrene; a 
      number of substrates for devices are, therefore, practical options. Oxidation of 
      the PDMS has the additional advantage that it yields channels whose walls are 
      negatively charged when in contact with neutral and basic aqueous solutions; 
      these channels support electroosmotic pumping and can be filled easily with 
      liquids with high surface energies (especially water). The performance of 
      microfluidic systems prepared using this rapid prototyping technique has been 
      evaluated by fabricating a miniaturized capillary electrophoresis system. Amino 
      acids, charge ladders of positively and negatively charged proteins, and DNA 
      fragments were separated in aqueous solutions with this system with resolution 
      comparable to that obtained using fused silica capillaries.
FAU - Duffy, D C
AU  - Duffy DC
AD  - Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford 
      Street, Cambridge, Massachusetts 02138.
FAU - McDonald, J C
AU  - McDonald JC
FAU - Schueller, O J
AU  - Schueller OJ
FAU - Whitesides, G M
AU  - Whitesides GM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
EDAT- 1998/12/01 00:00
MHDA- 1998/12/01 00:01
CRDT- 2011/06/08 06:00
PHST- 2011/06/08 06:00 [entrez]
PHST- 1998/12/01 00:00 [pubmed]
PHST- 1998/12/01 00:01 [medline]
AID - 10.1021/ac980656z [doi]
PST - ppublish
SO  - Anal Chem. 1998 Dec 1;70(23):4974-84. doi: 10.1021/ac980656z.