PMID- 21645311
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1753-6561 (Electronic)
IS  - 1753-6561 (Linking)
VI  - 5 Suppl 4
IP  - Suppl 4
DP  - 2011 Jun 3
TI  - Probing genetic control of swine responses to PRRSV infection: current progress 
      of the PRRS host genetics consortium.
PG  - S30
LID - 10.1186/1753-6561-5-S4-S30 [doi]
AB  - BACKGROUND: Understanding the role of host genetics in resistance to porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection, and the effects of 
      PRRS on pig health and related growth, are goals of the PRRS Host Genetics 
      Consortium (PHGC). METHODS: The project uses a nursery pig model to assess pig 
      resistance/susceptibility to primary PRRSV infection. To date, 6 groups of 200 
      crossbred pigs from high health farms were donated by commercial sources. After 
      acclimation, the pigs were infected with PRRSV in a biosecure facility and 
      followed for 42 days post infection (dpi). Blood samples were collected at 0, 4, 
      7, 10, 14, 21, 28, 35 and 42 dpi for serum and whole blood RNA gene expression 
      analyses; weekly weights were recorded for growth traits. All data have been 
      entered into the PHGC relational database. Genomic DNAs from all PHGC1-6 pigs 
      were prepared and genotyped with the Porcine SNP60 SNPchip. RESULTS: Results have 
      affirmed that all challenged pigs become PRRSV infected with peak viremia being 
      observed between 4-21 dpi. Multivariate statistical analyses of viral load and 
      weight data have identified PHGC pigs in different virus/weight categories. Sera 
      are now being compared for factors involved in recovery from infection, including 
      speed of response and levels of immune cytokines. Genome-wide association studies 
      (GWAS) are underway to identify genes and chromosomal locations that identify 
      PRRS resistant/susceptible pigs and pigs able to maintain growth while infected 
      with PRRSV. CONCLUSIONS: Overall, the PHGC project will enable researchers to 
      discover and verify important genotypes and phenotypes that predict 
      resistance/susceptibility to PRRSV infection. The availability of PHGC samples 
      provides a unique opportunity to continue to develop deeper phenotypes on every 
      PRRSV infected pig.
FAU - Lunney, Joan K
AU  - Lunney JK
AD  - Animal Parasitic Diseases Laboratory, BARC, ARS, USDA, Beltsville, MD 20705, USA. 
      Joan.Lunney@ars.usda.gov.
FAU - Steibel, Juan Pedro
AU  - Steibel JP
FAU - Reecy, James M
AU  - Reecy JM
FAU - Fritz, Eric
AU  - Fritz E
FAU - Rothschild, Max F
AU  - Rothschild MF
FAU - Kerrigan, Maureen
AU  - Kerrigan M
FAU - Trible, B
AU  - Trible B
FAU - Rowland, Raymond Rr
AU  - Rowland RR
LA  - eng
PT  - Journal Article
DEP - 20110603
PL  - England
TA  - BMC Proc
JT  - BMC proceedings
JID - 101316936
PMC - PMC3108226
EDAT- 2011/06/08 06:00
MHDA- 2011/06/08 06:01
PMCR- 2011/06/03
CRDT- 2011/06/08 06:00
PHST- 2011/06/08 06:00 [entrez]
PHST- 2011/06/08 06:00 [pubmed]
PHST- 2011/06/08 06:01 [medline]
PHST- 2011/06/03 00:00 [pmc-release]
AID - 1753-6561-5-S4-S30 [pii]
AID - 10.1186/1753-6561-5-S4-S30 [doi]
PST - epublish
SO  - BMC Proc. 2011 Jun 3;5 Suppl 4(Suppl 4):S30. doi: 10.1186/1753-6561-5-S4-S30.