PMID- 21645891
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20211020
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 96
IP  - 2
DP  - 2011 Aug
TI  - Reduced neurotrophin receptor tropomyosin-related kinase A expression in human 
      granulosa cells: a novel marker of diminishing ovarian reserve.
PG  - 474-478.e4
LID - 10.1016/j.fertnstert.2011.05.017 [doi]
AB  - OBJECTIVE: To determine whether human mural and cumulus granulosa cell 
      neurotrophin and neurotrophin receptor content correlate to ovarian reserve 
      markers. DESIGN: Prospective, laboratory-based study. SETTING: Academic assisted 
      reproductive technology (ART) program. PATIENT(S): Twenty-three women undergoing 
      ART. INTERVENTION(S): Mural and cumulus granulosa cells were collected from women 
      undergoing oocyte retrieval during ART cycles. Relative messenger RNA (mRNA) 
      levels of neurotrophins and their receptors were measured by quantitative reverse 
      transcription-polymerase chain reaction and correlated to serum antimullerian 
      hormone (AMH) levels and the number of oocytes retrieved. MAIN OUTCOME 
      MEASURE(S): Number of oocytes retrieved. RESULT(S): Mural and cumulus granulosa 
      cell nerve growth factor receptor tropomyosin-related kinase A (TrkA) mRNA 
      correlated strongly to the number of oocytes retrieved. Similarly, higher serum 
      AMH was associated with higher cumulus granulosa cell TrkA mRNA. Both mural and 
      cumulus granulosa cell p75(NTR)/TrkA ratios were lower in women with higher serum 
      AMH, and the number of oocytes retrieved was greater among women with low 
      p75(NTR)/TrkA ratio. No significant associations were found between brain-derived 
      neurotrophic factor (BDNF) and its specific receptor tropomyosin-related kinase B 
      (TrkB) and ovarian reserve markers. Although BDNF and TrkB expression were higher 
      in cumulus compared with mural granulosa cells, no such association was found 
      between TrkA and granulosa cells. Antimullerian hormone and cumulus TrkA mRNA, in 
      a model incorporating both, correlated strongly to the number of oocytes 
      retrieved (R(2) = 0.84). CONCLUSION(S): Cumulus TrkA and p75(NTR) mRNA correlate 
      to ovarian reserve, whereas BDNF and TrkB are associated with the type of 
      granulosa cell.
CI  - Copyright (c) 2011 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Buyuk, Erkan
AU  - Buyuk E
AD  - Division of Reproductive Endocrinology and Infertility, Department of Obstetrics 
      and Gynecology, Montefiore Institute for Reproductive Medicine and Health, Albert 
      Einstein College of Medicine, Bronx, New York 10461, USA. erbuyuk@yahoo.com
FAU - Santoro, Nanette
AU  - Santoro N
FAU - Cohen, Hillel W
AU  - Cohen HW
FAU - Charron, Maureen J
AU  - Charron MJ
FAU - Jindal, Sangita
AU  - Jindal S
LA  - eng
GR  - KL2 RR025749-01/RR/NCRR NIH HHS/United States
GR  - KL2 RR025749/RR/NCRR NIH HHS/United States
GR  - UL1 RR025750/RR/NCRR NIH HHS/United States
GR  - TL1 RR025748/RR/NCRR NIH HHS/United States
GR  - UL1 RR025750-03/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110608
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Genetic Markers)
RN  - 0 (NGF protein, human)
RN  - 0 (NGFR protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 80497-65-0 (Anti-Mullerian Hormone)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Adult
MH  - Anti-Mullerian Hormone/blood
MH  - Biomarkers/blood
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Down-Regulation
MH  - Female
MH  - Genetic Markers
MH  - Granulosa Cells/*enzymology
MH  - Humans
MH  - Nerve Growth Factor/genetics
MH  - Nerve Tissue Proteins/genetics
MH  - *Oocyte Retrieval
MH  - Ovary/*enzymology/physiopathology
MH  - Prospective Studies
MH  - RNA, Messenger/metabolism
MH  - Receptor, trkA/*genetics
MH  - Receptor, trkB/genetics
MH  - Receptors, Nerve Growth Factor/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3143233
MID - NIHMS296275
COIS- None of the authors have any conflict of interest to disclose.
EDAT- 2011/06/08 06:00
MHDA- 2011/09/29 06:00
PMCR- 2012/08/01
CRDT- 2011/06/08 06:00
PHST- 2011/03/25 00:00 [received]
PHST- 2011/05/06 00:00 [revised]
PHST- 2011/05/06 00:00 [accepted]
PHST- 2011/06/08 06:00 [entrez]
PHST- 2011/06/08 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - S0015-0282(11)00776-X [pii]
AID - 10.1016/j.fertnstert.2011.05.017 [doi]
PST - ppublish
SO  - Fertil Steril. 2011 Aug;96(2):474-478.e4. doi: 10.1016/j.fertnstert.2011.05.017. 
      Epub 2011 Jun 8.