PMID- 21646346
OWN - NLM
STAT- MEDLINE
DCOM- 20110928
LR  - 20211020
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 286
IP  - 30
DP  - 2011 Jul 29
TI  - MicroRNA expression profiles of human blood monocyte-derived dendritic cells and 
      macrophages reveal miR-511 as putative positive regulator of Toll-like receptor 
      4.
PG  - 26487-95
LID - 10.1074/jbc.M110.213561 [doi]
AB  - Dendritic cells (DCs) and macrophages (MFs) are important multifunctional immune 
      cells. Like other cell types, they express hundreds of different microRNAs 
      (miRNAs) that are recently discovered post-transcriptional regulators of gene 
      expression. Here we present updated miRNA expression profiles of monocytes, DCs 
      and MFs. Compared with monocytes,  approximately 50 miRNAs were found to be differentially 
      expressed in immature and mature DCs or MFs, with major expression changes 
      occurring during the differentiation. Knockdown of DICER1, a protein needed for 
      miRNA biosynthesis, led to lower DC-specific intercellular adhesion 
      molecule-3-grabbing non-integrin (DC-SIGN) and enhanced CD14 protein levels, 
      confirming the importance of miRNAs in DC differentiation in general. Inhibition 
      of the two most highly up-regulated miRNAs, miR-511 and miR-99b, also resulted in 
      reduced DC-SIGN level. Prediction of miRNA-511 targets revealed a number of genes 
      with known immune functions, of which TLR4 and CD80 were validated using 
      inhibition of miR-511 in DCs and luciferase assays in HEK293 cells. 
      Interestingly, under the cell cycle arrest conditions, miR-511 seems to function 
      as a positive regulator of TLR4. In conclusion, we have identified miR-511 as a 
      novel potent modulator of human immune response. In addition, our data highlight 
      that miRNA influence on gene expression is dependent on the cellular environment.
FAU - Tserel, Liina
AU  - Tserel L
AD  - Molecular Pathology, Faculty of Medicine, University of Tartu, Tartu 50411, 
      Estonia.
FAU - Runnel, Toomas
AU  - Runnel T
FAU - Kisand, Kai
AU  - Kisand K
FAU - Pihlap, Maire
AU  - Pihlap M
FAU - Bakhoff, Lairi
AU  - Bakhoff L
FAU - Kolde, Raivo
AU  - Kolde R
FAU - Peterson, Hedi
AU  - Peterson H
FAU - Vilo, Jaak
AU  - Vilo J
FAU - Peterson, Part
AU  - Peterson P
FAU - Rebane, Ana
AU  - Rebane A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110606
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (B7-1 Antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - EC 3.1.26.3 (DICER1 protein, human)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - B7-1 Antigen/genetics/immunology/metabolism
MH  - Cell Adhesion Molecules/genetics/immunology/metabolism
MH  - Cell Differentiation/physiology
MH  - DEAD-box RNA Helicases/genetics/immunology/metabolism
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*physiology
MH  - Gene Knockdown Techniques
MH  - HEK293 Cells
MH  - Humans
MH  - Lectins, C-Type/genetics/immunology/metabolism
MH  - Lipopolysaccharide Receptors/genetics/immunology/metabolism
MH  - Macrophages/cytology/immunology/*metabolism
MH  - MicroRNAs/*biosynthesis/genetics/immunology/metabolism
MH  - Monocytes/cytology/immunology/*metabolism
MH  - Receptors, Cell Surface/genetics/immunology/metabolism
MH  - Ribonuclease III/genetics/immunology/metabolism
MH  - Toll-Like Receptor 4/*biosynthesis/genetics/immunology
PMC - PMC3143613
EDAT- 2011/06/08 06:00
MHDA- 2011/09/29 06:00
PMCR- 2012/07/29
CRDT- 2011/06/08 06:00
PHST- 2011/06/08 06:00 [entrez]
PHST- 2011/06/08 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
PHST- 2012/07/29 00:00 [pmc-release]
AID - S0021-9258(19)48470-6 [pii]
AID - M110.213561 [pii]
AID - 10.1074/jbc.M110.213561 [doi]
PST - ppublish
SO  - J Biol Chem. 2011 Jul 29;286(30):26487-95. doi: 10.1074/jbc.M110.213561. Epub 
      2011 Jun 6.