PMID- 2164635
OWN - NLM
STAT- MEDLINE
DCOM- 19900822
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 10
IP  - 8
DP  - 1990 Aug
TI  - Lipopolysaccharide and dexamethasone induce mouse mammary tumor proviral gene 
      expression and differentiation in B lymphocytes through distinct regulatory 
      pathways.
PG  - 4211-20
AB  - Endogenous mouse mammary tumor virus (MMTV) proviral transcripts are up regulated 
      during the normal course of B-lymphocyte differentiation. We report here that the 
      regulatory mechanisms which lead to increased levels of MMTV transcripts in 
      differentiating, lipopolysaccharide (LPS)-stimulated normal B cells and in the 
      inducible B-cell lymphoma line CH12 are at least partially distinct from those 
      controlling increases in immunoglobulin and J-chain gene expression. In studies 
      designed to characterize the stimulatory pathways leading to MMTV expression in 
      CH12 cells, we found that stimulation with either LPS or dexamethasone (Dex), a 
      transcriptional activator of MMTV genes, induced not only MMTV expression but 
      also differentiation to antibody secretion. Only Dex-induced and not LPS-induced 
      MMTV expression and differentiation were inhibited by the glucocorticoid 
      antagonist RU486, demonstrating that Dex and LPS stimulate B cells by distinct 
      molecular pathways. Therefore, in B cells, MMTV expression can be regulated via 
      either the conventional hormone receptor-dependent pathway or a hormone 
      receptor-independent pathway. Furthermore, these results suggest that steroid 
      stimulation of B cells can lead to alterations in the expression of other results 
      suggest that steroid stimulation of B cells can lead to alterations in the 
      expression of other steroid-responsive genes that can become involved in the 
      process of B-cell differentiation.
FAU - King, L B
AU  - King LB
AD  - Department of Microbiology and Immunology, Duke Medical Center, Durham, North 
      Carolina 27710.
FAU - Corley, R B
AU  - Corley RB
LA  - eng
GR  - CA36642/CA/NCI NIH HHS/United States
GR  - T32 CA09058/CA/NCI NIH HHS/United States
GR  - T32 GM07184/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Immunoglobulin mu-Chains)
RN  - 0 (Lipopolysaccharides)
RN  - 320T6RNW1F (Mifepristone)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/cytology/drug effects/*immunology
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Transformation, Viral
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression/*drug effects
MH  - Gene Expression Regulation, Viral
MH  - Genes, Immunoglobulin/drug effects
MH  - Immunoglobulin mu-Chains/genetics
MH  - Kinetics
MH  - Lipopolysaccharides/*pharmacology
MH  - Mammary Tumor Virus, Mouse/drug effects/*genetics
MH  - Mice
MH  - Mifepristone/pharmacology
MH  - Proviruses/drug effects/*genetics
MH  - Transcription, Genetic/drug effects
PMC - PMC360955
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
PMCR- 1990/08/01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
PHST- 1990/08/01 00:00 [pmc-release]
AID - 10.1128/mcb.10.8.4211-4220.1990 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1990 Aug;10(8):4211-20. doi: 10.1128/mcb.10.8.4211-4220.1990.