PMID- 21646749
OWN - NLM
STAT- MEDLINE
DCOM- 20120529
LR  - 20190606
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Linking)
VI  - 73
IP  - 10
DP  - 2011 Oct
TI  - Possible origin of CSF antibodies induced by intrathecal immunization and 
      prophylactic effects against intracerebral rabies virus infection.
PG  - 1303-8
AB  - Intrathecal (IT) immunization involves injecting antigens directly into the 
      intraventricular or subarachnoid spaces, or brain, to induce antigen-specific 
      antibodies (Ab) in the cerebrospinal fluid (CSF). In the present study, rabbits 
      were immunized IT with inactivated rabies virus to investigate the origins of CSF 
      Ab. The time course of Ab induction and tumor necrosis factor-alpha expression 
      suggested the possibility that the CSF Ab originated in the serum. In addition, 
      Ab-producing cells infiltrated around the blood vessels of the brain, suggesting 
      local production of Ab within the central nervous system (CNS). Furthermore, 
      subcutaneous (SC) immunization prior to IT immunization induced a rapid and 
      magnified Ab response in the CSF compared with IT immunization alone. These 
      results were confirmed by the fact that mice immunized SC prior to IT were more 
      resistant to intracerebral challenge with rabies virus than mice immunized via 
      the IT route alone. Taken together, these results suggest that combined SC and IT 
      immunization is a more effective vaccination protocol for prophylaxis and 
      treatment of rabies.
FAU - Aoshima, Keisuke
AU  - Aoshima K
AD  - Laboratory of Comparative Pathology, Department of Veterinary Clinical Sciences, 
      School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
FAU - Sunden, Yuji
AU  - Sunden Y
FAU - Ishida, Sachiyo
AU  - Ishida S
FAU - Ochiai, Kenji
AU  - Ochiai K
FAU - Umemura, Takashi
AU  - Umemura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110607
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Viral)
RN  - 0 (Rabies Vaccines)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood/*cerebrospinal fluid
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Injections, Spinal
MH  - Injections, Subcutaneous
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Rabbits
MH  - Rabies/*immunology/metabolism/prevention & control
MH  - Rabies Vaccines/administration & dosage/*immunology
MH  - Rabies virus/*immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vaccination/*methods
EDAT- 2011/06/08 06:00
MHDA- 2012/05/30 06:00
CRDT- 2011/06/08 06:00
PHST- 2011/06/08 06:00 [entrez]
PHST- 2011/06/08 06:00 [pubmed]
PHST- 2012/05/30 06:00 [medline]
AID - JST.JSTAGE/jvms/11-0183 [pii]
AID - 10.1292/jvms.11-0183 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2011 Oct;73(10):1303-8. doi: 10.1292/jvms.11-0183. Epub 2011 Jun 
      7.