PMID- 21647406
OWN - NLM
STAT- MEDLINE
DCOM- 20111027
LR  - 20211020
IS  - 1740-2530 (Electronic)
IS  - 1740-2522 (Print)
IS  - 1740-2522 (Linking)
VI  - 2011
DP  - 2011
TI  - IFN regulatory factors 4 and 8 expression in the NOD mouse.
PG  - 374859
LID - 10.1155/2011/374859 [doi]
LID - 374859
AB  - Dendritic cells (DCs) contribute to islet inflammation and its progression to 
      diabetes in NOD mouse model and human. DCs play a crucial role in the 
      presentation of autoantigen and activation of diabetogenic T cells, and IRF4 and 
      IRF8 are crucial genes involved in the development of DCs. We have therefore 
      investigated the expression of these genes in splenic DCs during diabetes 
      progression in NOD mice. We found that IRF4 expression was upregulated in 
      splenocytes and in splenic CD11c(+) DCs of NOD mice as compared to BALB/c mice. 
      In contrast, IRF8 gene expression was higher in splenocytes of NOD mice whereas 
      its expression was similar in splenic CD11c(+) DCs of NOD and BALB/c mice. 
      Importantly, levels of IRF4 and IRF8 expression were lower in tolerogenic bone 
      marrow derived DCs (BMDCs) generated with GM-CSF as compared to immunogenic BMDCs 
      generated with GM-CSF and IL-4. Analysis of splenic DCs subsets indicated that 
      high expression of IRF4 was associated with increased levels of 
      CD4(+)CD8alpha(-)IRF4(+)CD11c(+) DCs but not CD4(-)CD8alpha(+)IRF8(+)CD11c(+) DCs in NOD 
      mice. Our results showed that IRF4 expression was up-regulated in NOD mice and 
      correlated with the increased levels of CD4(+)CD8alpha(-) DCs, suggesting that IRF4 
      may be involved in abnormal DC functions in type 1 diabetes in NOD mice.
FAU - Besin, Gilles
AU  - Besin G
AD  - Department of Pediatric, Immunology Division, Faculty of Medicine and Health 
      Sciences, University of Sherbrooke, 3001 12th Avenue North, Sherbrooke, QC, 
      Canada.
FAU - Gaudreau, Simon
AU  - Gaudreau S
FAU - Dumont-Blanchette, Emilie
AU  - Dumont-Blanchette E
FAU - Menard, Michael
AU  - Menard M
FAU - Guindi, Chantal
AU  - Guindi C
FAU - Dupuis, Gilles
AU  - Dupuis G
FAU - Amrani, Abdelaziz
AU  - Amrani A
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110515
PL  - Egypt
TA  - Clin Dev Immunol
JT  - Clinical & developmental immunology
JID - 101183692
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (interferon regulatory factor-4)
RN  - 0 (interferon regulatory factor-8)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Interferon Regulatory Factors/*genetics/metabolism
MH  - Interleukin-4/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred NOD
MH  - Up-Regulation
PMC - PMC3102445
EDAT- 2011/06/08 06:00
MHDA- 2011/10/28 06:00
PMCR- 2011/05/15
CRDT- 2011/06/08 06:00
PHST- 2011/01/17 00:00 [received]
PHST- 2011/03/09 00:00 [accepted]
PHST- 2011/06/08 06:00 [entrez]
PHST- 2011/06/08 06:00 [pubmed]
PHST- 2011/10/28 06:00 [medline]
PHST- 2011/05/15 00:00 [pmc-release]
AID - 10.1155/2011/374859 [doi]
PST - ppublish
SO  - Clin Dev Immunol. 2011;2011:374859. doi: 10.1155/2011/374859. Epub 2011 May 15.