PMID- 21648284
OWN - NLM
STAT- MEDLINE
DCOM- 20110705
LR  - 20190907
IS  - 0334-018X (Print)
IS  - 0334-018X (Linking)
VI  - 24
IP  - 3-4
DP  - 2011
TI  - Evidence of insulin-like growth factor binding protein-3 proteolysis during 
      growth hormone stimulation testing.
PG  - 163-7
AB  - OBJECTIVES: The ternary complex is composed of insulin-like growth factor 
      (IGF)-I, IGF binding protein (IGFBP)-3 and acid labile subunit (ALS). Growth 
      hormone (GH) promotes IGFBP-3 proteolysis to release free IGF-I, ALS, and IGFBP-3 
      fragments. Our aim was to determine whether elevated GH levels during GH 
      stimulation testing would trigger IGFBP-3 proteolysis. DESIGN: This prospective 
      study of 10 short prepubertal children (height standard deviation score -2.37 +/- 
      0.31) used arginine and GH releasing hormone stimulation to study dynamic changes 
      in the ternary complex moieties. IGFBP-3 was measured in two assays: a 
      radioimmunoassay (RIA) that detects both cleaved and intact IGFBP-3; and an 
      immunochemiluminescence assay (ICMA) that detects only intact IGFBP-3. RESULTS: 
      IGFBP-3 measured by RIA increased by 19% (p < 0.05), while IGFBP-3 measured by 
      ICMA did not significantly increase (6.1%). CONCLUSION: The significant increase 
      in IGFBP-3 measured by RIA, but not ICMA, provides evidence of IGFBP-3 
      proteolysis during acute GH stimulation.
FAU - Nwosu, Benjamin U
AU  - Nwosu BU
AD  - Division of Endocrinology, Department of Pediatrics, University of Massachusetts 
      Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. 
      benjamin.nwosu@umassmemorial.org
FAU - Soyka, Leslie A
AU  - Soyka LA
FAU - Angelescu, Amanda
AU  - Angelescu A
FAU - Lee, Mary M
AU  - Lee MM
LA  - eng
SI  - ClinicalTrials.gov/NCT00235599
GR  - DK32520/DK/NIDDK NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Pediatr Endocrinol Metab
JT  - Journal of pediatric endocrinology & metabolism : JPEM
JID - 9508900
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - 12629-01-5 (Human Growth Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Arginine/blood
MH  - Child
MH  - Dwarfism/*drug therapy/metabolism
MH  - Female
MH  - Growth Hormone-Releasing Hormone/*therapeutic use
MH  - Human Growth Hormone/*metabolism
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Protein 3/*blood
MH  - Male
MH  - Prospective Studies
MH  - Puberty
EDAT- 2011/06/09 06:00
MHDA- 2011/07/06 06:00
CRDT- 2011/06/09 06:00
PHST- 2011/06/09 06:00 [entrez]
PHST- 2011/06/09 06:00 [pubmed]
PHST- 2011/07/06 06:00 [medline]
AID - 10.1515/jpem.2011.088 [doi]
PST - ppublish
SO  - J Pediatr Endocrinol Metab. 2011;24(3-4):163-7. doi: 10.1515/jpem.2011.088.