PMID- 21651861
OWN - NLM
STAT- MEDLINE
DCOM- 20110922
LR  - 20111117
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 27
IP  - 6
DP  - 2011 Jun
TI  - [CTLs induced by hTERT-related multiepitope peptide-sensitived mDCs specifically 
      kill HLA-A24+ tumor cells].
PG  - 626-30
AB  - AIM: To study the antigen specific anti-tumor effect of cytotoxic T 
      lymphocytes(CTLs), which was induced by human telomerase reverse transcriptase 
      (hTERT)-related multiple epitope peptides impulsed myeloid dendritic cells(mDCs), 
      against human leukocyte antigen (HLA)-A24(+) tumor cells. METHODS: Four branches 
      of multiple antigen peptides (MAPs) of hTERT epitopes and three separate peptides 
      were solid-phase artificially synthesized, phlebotomize peripheral blood from 
      HLA-A24(+) healthy volunteers, sorted the blood through MACS MicroBeads and 
      cultured mDCs, Nylon fiber column purified T lymphocytes, mDCs impulsed with each 
      type of peptides were co-cultured with T lymphocytes to induce CTLs specifically 
      killing effect, and the resultant CTLs were used as effector cells, SMMC-7721 
      with hTERT and HLA-A24 positive and SKOV3 which are hTERT-positive but 
      HLA-A24-negative tumor cells were used as target cells. The level of human IL-12, 
      TNF-alpha in the culture supernatant was determined by ELISA. Flow cytometry assay 
      was used to assess the killing ability of CTLs against tumor cells. RESULTS: MAPs 
      of hTERT epitopes including I540 (ILAKFLHWL), V461 (VYGFVRACL), L766 
      (LTDLQPYMRQFVAHL) and three separate peptides could impulse mDCs and then induce 
      CTLs to specifically kill SMMC-7721, CTLs induced by MAPs had stronger cytotoxic 
      effect compared with three separate peptides mixed(P < 0.05). CONCLUSION: 
      mDCs-impulsed with hTERT-associated MAPs can induce production and proliferation 
      of allogenic CTLs, which show antigen specific anti-tumor effect against 
      HLA-A24(+) tumor cells. This result has significantly meaning in tumor 
      immunotherapy.
FAU - Shen, Hua-ping
AU  - Shen HP
AD  - Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400010, China.
FAU - Niu, Bo-lin
AU  - Niu BL
FAU - DU, Hui-min
AU  - DU HM
FAU - Zou, Li-quan
AU  - Zou LQ
FAU - Gong, Jian-ping
AU  - Gong JP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (Peptide Fragments)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Antigens, Neoplasm/immunology
MH  - Carcinoma, Hepatocellular/*immunology/therapy
MH  - Cell Death/*immunology
MH  - Cell Line, Tumor
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Epitopes/immunology
MH  - Female
MH  - HLA-A Antigens/metabolism
MH  - HLA-A24 Antigen
MH  - Humans
MH  - Immunotherapy
MH  - Interleukin-12/metabolism
MH  - Liver Neoplasms/*immunology/therapy
MH  - Peptide Fragments/*administration & dosage/chemical synthesis/immunology
MH  - T-Lymphocytes, Cytotoxic/drug effects/*immunology
MH  - Telomerase/*genetics
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2011/06/10 06:00
MHDA- 2011/09/23 06:00
CRDT- 2011/06/10 06:00
PHST- 2011/06/10 06:00 [entrez]
PHST- 2011/06/10 06:00 [pubmed]
PHST- 2011/09/23 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Jun;27(6):626-30.