PMID- 21652062
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20131121
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 76
IP  - 4
DP  - 2011 Sep 1
TI  - A simple method for producing tetraploid porcine parthenogenetic embryos.
PG  - 598-606
LID - 10.1016/j.theriogenology.2011.03.010 [doi]
AB  - The objective was to produce porcine tetraploid parthenogenetic embryos using 
      cytochalasin B, which inhibits polar body extrusion. Porcine cumulus-enclosed 
      oocytes aspirated from antral follicles were cultured for 51 h, and treated with 
      cytochalasin B from 35 h to 42 h after the start of culture. After maturation 
      culture, 74.7% (2074/2775) of oocytes treated with cytochalasin B did not extrude 
      a polar body (0PB oocytes). In contrast, 80.4% (1931/2403) of control oocytes 
      extruded a polar body (1PB oocytes). The 0PB oocytes were electrically 
      stimulated, treated with cytochalasin B again for 3 h, and then cultured without 
      cytochalasin B. Six days after electrical stimulation, 49.8% (321/644) reached 
      the blastocyst stage. The number of cells in these blastocysts derived from 0PB 
      oocytes was significantly lower than that from 1PB oocytes (0PB: 24.9 +/- 10.6; 
      1PB: 43.0 +/- 17.1; mean +/- SD). A porcine chromosome 1-specific sequence was 
      detected in parthenogenetic 0PB embryos by fluorescence in situ hybridization 
      (FISH) analysis. Typical pronucleus-stage samples derived from 0PB embryos had 
      two pronuclei, each with two signals. In two-cell and blastocyst-stage embryos, 
      four signals were detected in each nucleus derived from 0PB embryos. We inferred 
      that 0PB oocytes, which had a tetraploid number of chromosomes, started to 
      develop as tetraploid parthenotes after electrical stimulation, and that 
      tetraploid status was stably maintained during early embryonic development, at 
      least until the blastocyst stage.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Sembon, S
AU  - Sembon S
AD  - Transgenic Animal Research Center, National Institute of Agrobiological Sciences, 
      Ibaraki, Japan. senbon@affrc.go.jp
FAU - Fuchimoto, D
AU  - Fuchimoto D
FAU - Iwamoto, M
AU  - Iwamoto M
FAU - Suzuki, S
AU  - Suzuki S
FAU - Yoshioka, K
AU  - Yoshioka K
FAU - Onishi, A
AU  - Onishi A
LA  - eng
PT  - Journal Article
DEP - 20110608
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 3CHI920QS7 (Cytochalasin B)
SB  - IM
MH  - Animals
MH  - Chi-Square Distribution
MH  - Cytochalasin B/*pharmacology
MH  - Electric Stimulation/methods
MH  - Embryo Culture Techniques/methods/*veterinary
MH  - Embryonic Development/physiology
MH  - Female
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Meiosis/physiology
MH  - Oocytes/cytology/*physiology
MH  - Parthenogenesis/*physiology
MH  - Pregnancy
MH  - Swine/embryology/genetics/*physiology
MH  - *Tetraploidy
EDAT- 2011/06/10 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/06/10 06:00
PHST- 2010/10/07 00:00 [received]
PHST- 2011/03/15 00:00 [revised]
PHST- 2011/03/15 00:00 [accepted]
PHST- 2011/06/10 06:00 [entrez]
PHST- 2011/06/10 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - S0093-691X(11)00146-4 [pii]
AID - 10.1016/j.theriogenology.2011.03.010 [doi]
PST - ppublish
SO  - Theriogenology. 2011 Sep 1;76(4):598-606. doi: 
      10.1016/j.theriogenology.2011.03.010. Epub 2011 Jun 8.