PMID- 21652138
OWN - NLM
STAT- MEDLINE
DCOM- 20120726
LR  - 20171116
IS  - 1578-1267 (Electronic)
IS  - 0301-0546 (Linking)
VI  - 40
IP  - 2
DP  - 2012 Mar-Apr
TI  - T-cell proliferation by surface molecules expression on polymorphonuclear 
      neutrophils stimulated with IL-4 in superantigen presence.
PG  - 81-7
LID - 10.1016/j.aller.2011.02.012 [doi]
AB  - BACKGROUND: Polymorphonuclear neutrophils (PMNs) were originally described as 
      short lived and terminally differentiated phagocytes that contribute only to the 
      innate immune response. Some studies of PMNs cytokine production and expression 
      of numerous cell surface proteins has suggested that PMNs are likely to influence 
      adaptive responses and may satisfy the criteria of antigen presenting cells. AIM 
      OF THE STUDY: This work aimed to study the effect of IL-4 in the function of PMNs 
      as antigen presenting cells. METHODS: Flow cytometry was used in the present 
      study for the detection of cell surface human leukocyte antigen (HLA) class II, 
      CD80 and CD86 required for antigen presentation and subsequent T-cell activation 
      in the presence of Staphylococcus aureus enterotoxin (A). Human peripheral blood 
      neutrophils were used for this purpose. RESULTS: This study has shown that IL-4 
      stimulated PMNs for 24h expressed HLA class II, CD80 and CD86 that involved in 
      antigen presentation. It also indicated that co-cultivation of IL-4 stimulated 
      PMNs with autologous T-cells and in the presence of S. aureus enterotoxin (A) 
      induced T-cell proliferation. CONCLUSIONS: In vitro stimulation of PMNs with IL-4 
      showed expression of surface molecules involved in antigen presentation. In 
      addition, the co-culture of T-Cells and stimulated PMNs showed high T-Cells 
      proliferation in the presence of superantigens.
CI  - Copyright A(c) 2010 SEICAP. Published by Elsevier Espana. All rights reserved.
FAU - Abdel-Salam, B K A
AU  - Abdel-Salam BK
AD  - Department of Zoology, Minia University, El-Minia, Egypt. Manar_Muhamad@yahoo.com
LA  - eng
PT  - Journal Article
DEP - 20110608
PL  - Singapore
TA  - Allergol Immunopathol (Madr)
JT  - Allergologia et immunopathologia
JID - 0370073
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Enterotoxins)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Superantigens)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37337-57-8 (enterotoxin A, Staphylococcal)
SB  - IM
MH  - Antigen Presentation
MH  - B7-1 Antigen/metabolism
MH  - B7-2 Antigen/metabolism
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Enterotoxins/immunology/*metabolism
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Humans
MH  - Interleukin-4/immunology/metabolism
MH  - Lymphocyte Activation
MH  - Neutrophils/cytology/immunology/*metabolism
MH  - Superantigens/immunology/*metabolism
MH  - T-Lymphocytes/immunology/*metabolism/pathology
EDAT- 2011/06/10 06:00
MHDA- 2012/07/27 06:00
CRDT- 2011/06/10 06:00
PHST- 2010/12/28 00:00 [received]
PHST- 2011/02/12 00:00 [revised]
PHST- 2011/02/14 00:00 [accepted]
PHST- 2011/06/10 06:00 [entrez]
PHST- 2011/06/10 06:00 [pubmed]
PHST- 2012/07/27 06:00 [medline]
AID - S0301-0546(11)00156-X [pii]
AID - 10.1016/j.aller.2011.02.012 [doi]
PST - ppublish
SO  - Allergol Immunopathol (Madr). 2012 Mar-Apr;40(2):81-7. doi: 
      10.1016/j.aller.2011.02.012. Epub 2011 Jun 8.