PMID- 21653745
OWN - NLM
STAT- MEDLINE
DCOM- 20111121
LR  - 20211020
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 18
IP  - 8
DP  - 2011 Aug
TI  - A live-attenuated chimeric porcine circovirus type 2 (PCV2) vaccine is 
      transmitted to contact pigs but is not upregulated by concurrent infection with 
      porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus 
      (PRRSV) and is efficacious in a PCV2b-PRRSV-PPV challenge model.
PG  - 1261-8
LID - 10.1128/CVI.05057-11 [doi]
AB  - The live chimeric porcine circovirus type 2 (PCV2) vaccine with the capsid gene 
      of the emerging subtype 2b cloned in the genomic backbone of the nonpathogenic 
      PCV1 is attenuated in vivo and induces protective immunity against PCV2. To 
      further determine the safety and efficacy of this experimental vaccine, we tested 
      for evidence of pig-to-pig transmission by commingling nonvaccinated and 
      vaccinated pigs, determined potential upregulation by simultaneous vaccination 
      and infection with porcine parvovirus (PPV) and porcine reproductive and 
      respiratory syndrome virus (PRRSV), and determined vaccine efficacy by 
      challenging pigs 4 weeks after vaccination with PCV2b, PRRSV, and PPV. Forty-six 
      21-day-old, PCV2-naive pigs were randomly assigned to one of six groups. 
      Twenty-nine of 46 pigs were challenged with PCV2b, PRRSV, and PPV at day 28, 8/46 
      remained nonvaccinated and nonchallenged and served as negative controls, and 
      9/46 remained nonchallenged and served as vaccination controls. All animals were 
      necropsied at day 49. PCV1-PCV2 viremia was detected in nonvaccinated contact 
      pigs commingled with vaccinated pigs, indicating pig-to-pig transmission; 
      however, PCV1-PCV2 DNA levels remained low in all vaccinated and contact pigs 
      regardless of concurrent infection. Finally, vaccination 28 days before challenge 
      resulted in significantly (P < 0.05) decreased amounts of PCV2 in tissues and 
      sera and significantly (P < 0.05) reduced macroscopic and microscopic lesions. 
      The results of this study indicate that the experimental live-attenuated chimeric 
      PCV2 vaccine, although transmissible to contact pigs, remains attenuated in pigs 
      concurrently infected with PRRSV and PPV and induces protective immunity against 
      PCV2b when it is administered 28 days before PCV2 exposure.
FAU - Opriessnig, T
AU  - Opriessnig T
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. 
      tanjaopr@iastate.edu
FAU - Shen, H G
AU  - Shen HG
FAU - Pal, N
AU  - Pal N
FAU - Ramamoorthy, S
AU  - Ramamoorthy S
FAU - Huang, Y W
AU  - Huang YW
FAU - Lager, K M
AU  - Lager KM
FAU - Beach, N M
AU  - Beach NM
FAU - Halbur, P G
AU  - Halbur PG
FAU - Meng, X J
AU  - Meng XJ
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20110608
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/immunology/prevention & control/*veterinary/virology
MH  - Circovirus/*immunology
MH  - Parvovirus, Porcine/immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Swine
MH  - Swine Diseases/immunology/*prevention & control/virology
MH  - Vaccines, Attenuated/administration & dosage/adverse effects/immunology
MH  - Viral Vaccines/administration & dosage/adverse effects/*immunology
PMC - PMC3147358
EDAT- 2011/06/10 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/02/01
CRDT- 2011/06/10 06:00
PHST- 2011/06/10 06:00 [entrez]
PHST- 2011/06/10 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/02/01 00:00 [pmc-release]
AID - CVI.05057-11 [pii]
AID - 5057-11 [pii]
AID - 10.1128/CVI.05057-11 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2011 Aug;18(8):1261-8. doi: 10.1128/CVI.05057-11. Epub 2011 
      Jun 8.