PMID- 21655296
OWN - NLM
STAT- MEDLINE
DCOM- 20111005
LR  - 20220129
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 5
DP  - 2011
TI  - Circulating vascular progenitor cells and central arterial stiffness in 
      polycystic ovary syndrome.
PG  - e20317
LID - 10.1371/journal.pone.0020317 [doi]
LID - e20317
AB  - OBJECTIVE: Subjects with Polycystic ovarian syndrome (PCOS) are at increased risk 
      of Type 2 diabetes mellitus (T2DM). The mechanism of this enhanced risk is 
      unclear. Circulating vascular progenitor cells (VPC) are immature bone marrow 
      derived cells capable of differentiating into mature endothelial cells. VPC 
      number/function and central arterial stiffness predict cardio-metabolic disease 
      in at-risk populations. DESIGN: We studied VPC and arterial stiffness measures in 
      non-obese PCOS subjects as compared to age and body mass index (BMI) matched 
      healthy controls in a cross-sectional study. METHODS: Fourteen subjects with PCOS 
      and 12 controls of similar age, BMI (all <30 kg/m(2)) and metabolic profile were 
      studied. VPC number and in vitro function were studied by flow cytometry and tube 
      formation assays respectively. Augmentation index (AIx), a measure of central 
      arterial stiffness, and central (aortic) blood pressures (BP) were measured by 
      applanation tonometry. RESULTS: Subjects with PCOS had a reduced number, 
      mean+/-SEM, of circulating CD34(+)133(+) VPCs (317.5+/-51.0 vs. 558.3+/-101.2, 
      p = 0.03) and impaired in vitro tube formation (completed tube area 1.0+/-0.06 vs. 
      1.2+/-0.05x10(6) microm(2) p = 0.02). PCOS subjects had significantly higher AIx 
      (18.4+/-1.9% vs. 4.9+/-2.0%) and this difference remained significant even after 
      adjustments for age, BMI and smoking (p = 0.003) in multivariate analyses. 
      Central systolic and pulse pressure were higher in PCOS subjects but these 
      differences were not statistically significant after adjustment for age. Brachial 
      systolic and pulse pressures were similar. VPC number/function and arterial 
      stiffness or BP measures were not correlated. CONCLUSIONS: Non-obese PCOS is 
      characterized by a reduced VPC number, impaired VPC function and increased 
      central arterial stiffness. These changes in novel vascular risk markers may 
      explain the enhanced risk of T2DM and CVD in PCOS.
FAU - Dessapt-Baradez, Cecile
AU  - Dessapt-Baradez C
AD  - Cardiovascular Division at Guy's and St Thomas and King's College Hospitals, 
      King's College London, London, United Kingdom. cecile.dessapt@kcl.ac.uk
FAU - Reza, Maria
AU  - Reza M
FAU - Sivakumar, Ghayathri
AU  - Sivakumar G
FAU - Hernandez-Fuentes, Maria
AU  - Hernandez-Fuentes M
FAU - Markakis, Kostas
AU  - Markakis K
FAU - Gnudi, Luigi
AU  - Gnudi L
FAU - Karalliedde, Janaka
AU  - Karalliedde J
LA  - eng
GR  - Wellcome Trust/United Kingdom
GR  - G0801537/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110531
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Glycoproteins)
RN  - 0 (Peptides)
SB  - IM
MH  - AC133 Antigen
MH  - Adult
MH  - Antigens, CD/metabolism
MH  - Antigens, CD34/metabolism
MH  - Arteries/physiopathology
MH  - Body Mass Index
MH  - Cross-Sectional Studies
MH  - Endothelial Cells/cytology/*physiology
MH  - Female
MH  - Glycoproteins/metabolism
MH  - Humans
MH  - Peptides/metabolism
MH  - Polycystic Ovary Syndrome/blood/*physiopathology
MH  - Stem Cells/cytology/*physiology
MH  - Young Adult
PMC - PMC3105021
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/06/10 06:00
MHDA- 2011/10/06 06:00
PMCR- 2011/05/31
CRDT- 2011/06/10 06:00
PHST- 2011/01/21 00:00 [received]
PHST- 2011/04/22 00:00 [accepted]
PHST- 2011/06/10 06:00 [entrez]
PHST- 2011/06/10 06:00 [pubmed]
PHST- 2011/10/06 06:00 [medline]
PHST- 2011/05/31 00:00 [pmc-release]
AID - PONE-D-11-01740 [pii]
AID - 10.1371/journal.pone.0020317 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(5):e20317. doi: 10.1371/journal.pone.0020317. Epub 2011 May 31.