PMID- 21655924 OWN - NLM STAT- MEDLINE DCOM- 20120731 LR - 20220409 IS - 1532-2807 (Electronic) IS - 1219-4956 (Linking) VI - 18 IP - 2 DP - 2012 Apr TI - p53 Binds to estrogen receptor 1 promoter in human breast cancer cells. PG - 169-75 LID - 10.1007/s12253-011-9423-6 [doi] AB - p53 is a tumor suppressor protein that regulates estrogen receptor 1 (ESR1) expression. To investigate the mechanism of ESR1 gene regulation by p53, chromatin immunoprecipitation was applied to assess the binding of p53, DNMT1, HDAC1 and MeCP2 to both silenced ESR1 promoter in MDA-MB-468 cells and active ESR1 promoter in MCF-7 breast cancer cells. The results of chromatin immunoprecipitation experiments showed that p53 protein binds to both unmethylated CpG island of the ESR1 promoter in the ER-positive MCF-7 and the hypermethylated ESR1 promoter in the ER-negative MDA-MB-468 cells. However, repression complex including DNMT1, HDAC1 and MeCP2 is only associated with silenced ESR1 in ER-negative MDA-MB-468 human breast cancer cells. In addition, ectopically expressed wild type p53 failed to reactivate the ESR1 gene in these cells. These results suggest that specific p53 mutations may contribute to loss of estrogen receptor alpha expression in breast tumors and also support the hypothesis that mutant p53 is likely to impact DNA methylation. FAU - Rasti, Mozhgan AU - Rasti M AD - Recombinant protein lab, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. rasti31@yahoo.com FAU - Arabsolghar, Rita AU - Arabsolghar R FAU - Khatooni, Zahed AU - Khatooni Z FAU - Mostafavi-Pour, Zoherh AU - Mostafavi-Pour Z LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110608 PL - Switzerland TA - Pathol Oncol Res JT - Pathology oncology research : POR JID - 9706087 RN - 0 (ESR1 protein, human) RN - 0 (Estrogen Receptor alpha) RN - 0 (MECP2 protein, human) RN - 0 (Methyl-CpG-Binding Protein 2) RN - 0 (RNA, Messenger) RN - 0 (TP53 protein, human) RN - 0 (Tumor Suppressor Protein p53) RN - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1) RN - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases) RN - EC 2.1.1.37 (DNMT1 protein, human) RN - EC 3.5.1.98 (HDAC1 protein, human) RN - EC 3.5.1.98 (Histone Deacetylase 1) SB - IM MH - Blotting, Western MH - Breast Neoplasms/*genetics/*metabolism/pathology MH - Chromatin Immunoprecipitation MH - CpG Islands MH - DNA (Cytosine-5-)-Methyltransferase 1 MH - DNA (Cytosine-5-)-Methyltransferases/genetics/metabolism MH - DNA Methylation MH - Estrogen Receptor alpha/*genetics MH - Female MH - *Gene Expression Regulation, Neoplastic MH - Histone Deacetylase 1/genetics/metabolism MH - Humans MH - Methyl-CpG-Binding Protein 2/genetics/metabolism MH - Mutation/genetics MH - Promoter Regions, Genetic/*genetics MH - RNA, Messenger/genetics MH - Real-Time Polymerase Chain Reaction MH - Regulatory Sequences, Nucleic Acid MH - Transcription, Genetic MH - Tumor Cells, Cultured MH - Tumor Suppressor Protein p53/*genetics/*metabolism EDAT- 2011/06/10 06:00 MHDA- 2012/08/01 06:00 CRDT- 2011/06/10 06:00 PHST- 2011/03/05 00:00 [received] PHST- 2011/05/30 00:00 [accepted] PHST- 2011/06/10 06:00 [entrez] PHST- 2011/06/10 06:00 [pubmed] PHST- 2012/08/01 06:00 [medline] AID - 10.1007/s12253-011-9423-6 [doi] PST - ppublish SO - Pathol Oncol Res. 2012 Apr;18(2):169-75. doi: 10.1007/s12253-011-9423-6. Epub 2011 Jun 8.