PMID- 21658996
OWN - NLM
STAT- MEDLINE
DCOM- 20120106
LR  - 20191210
IS  - 1618-0607 (Electronic)
IS  - 1438-4221 (Linking)
VI  - 301
IP  - 7
DP  - 2011 Nov
TI  - Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by 
      fluorescence in situ hybridization (FISH) from culture and paraffin-embedded 
      tissue samples.
PG  - 585-90
LID - 10.1016/j.ijmm.2011.04.017 [doi]
AB  - We evaluated newly developed probes for rapid identification of Burkholderia (B.) 
      pseudomallei and B. mallei and differentiation from B. thailandensis by 
      fluorescence in situ hybridization (FISH). FISH correctly identified 100% of the 
      tested B. pseudomallei (11), B. mallei (11), and B. thailandensis (1) strains, 
      excluded 100% of all tested negative controls (61), and allowed demonstration of 
      B. pseudomallei infection in a paraffin-embedded spleen tissue sample of an 
      experimentally infected mouse.
CI  - Copyright (c) 2011 Elsevier GmbH. All rights reserved.
FAU - Hagen, Ralf M
AU  - Hagen RM
AD  - Department for Tropical Medicine at the Bernhard Nocht Institute, Bundeswehr 
      Hospital Hamburg, Bernhard-Nocht-Strasse, Germany. hagen@bni-hamburg.de
FAU - Frickmann, Hagen
AU  - Frickmann H
FAU - Elschner, Mandy
AU  - Elschner M
FAU - Melzer, Falk
AU  - Melzer F
FAU - Neubauer, Heinrich
AU  - Neubauer H
FAU - Gauthier, Yves P
AU  - Gauthier YP
FAU - Racz, Paul
AU  - Racz P
FAU - Poppert, Sven
AU  - Poppert S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20110611
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Animals
MH  - Bacteriological Techniques/*methods
MH  - Burkholderia mallei/genetics/*isolation & purification
MH  - Burkholderia pseudomallei/genetics/*isolation & purification
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Oligonucleotide Probes/genetics
MH  - Pathology/*methods
MH  - Sensitivity and Specificity
MH  - Spleen/microbiology
EDAT- 2011/06/11 06:00
MHDA- 2012/01/10 06:00
CRDT- 2011/06/11 06:00
PHST- 2011/02/15 00:00 [received]
PHST- 2011/04/12 00:00 [revised]
PHST- 2011/04/20 00:00 [accepted]
PHST- 2011/06/11 06:00 [entrez]
PHST- 2011/06/11 06:00 [pubmed]
PHST- 2012/01/10 06:00 [medline]
AID - S1438-4221(11)00047-6 [pii]
AID - 10.1016/j.ijmm.2011.04.017 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2011 Nov;301(7):585-90. doi: 10.1016/j.ijmm.2011.04.017. 
      Epub 2011 Jun 11.