PMID- 21660599
OWN - NLM
STAT- MEDLINE
DCOM- 20120316
LR  - 20211020
IS  - 1573-3017 (Electronic)
IS  - 0963-9292 (Linking)
VI  - 20
IP  - 8
DP  - 2011 Nov
TI  - Transcriptomic analyses in a benthic fish exposed to contaminated estuarine 
      sediments through laboratory and in situ bioassays.
PG  - 1749-64
LID - 10.1007/s10646-011-0708-z [doi]
AB  - The transcription of contaminant response-related genes was investigated in 
      juvenile Senegalese soles exposed to sediments from three distinct sites (a 
      reference plus two contaminated) of a Portuguese estuary (the Sado, W Portugal) 
      through simultaneous 28-day laboratory and in situ bioassays. Transcription of 
      cytochrome P450 1A (CYP1A), metallothionein 1 (MT1), glutathione peroxidase 
      (GPx), catalase (CAT), caspase 3 (CASP3) and 90 kDa heat-shock protein alpha 
      (HSP90AA) was surveyed in the liver by real-time PCR. CASP3 transcription 
      analysis was complemented by surveying apoptosis through the TUNEL reaction. 
      After 14 days of exposure, relative transcription was either reduced or decreased 
      in fish exposed to the contaminated sediments, revealing a disturbance stress 
      phase during which animals failed to respond to insult. After 28 days of exposure 
      all genes' transcription responded to contamination but laboratory and in situ 
      assays depicted distinct patterns of regulation. Although sediments revealed a 
      combination of organic and inorganic toxicants, transcription of the CYP1A gene 
      was consistently correlated to organic contaminants. Metallothionein regulation 
      was found correlated to metallic and organic xenobiotic contamination in the 
      laboratory and in situ, respectively. The transcription of oxidative 
      stress-related genes can be a good indicator of general stress but caution is 
      mandatory when interpreting the results since regulation may be influenced by 
      multiple factors. As for MT1, HSP90 up-regulation has potential to be a good 
      indicator for total contamination, as well as the CASP3 gene, even though 
      hepatocyte apoptosis depicted values inconsistent with sediment contamination, 
      showing that programmed cell death did not directly depend on caspase 
      transcription alone.
FAU - Costa, Pedro M
AU  - Costa PM
AD  - IMAR-Instituto do Mar, Departamento de Ciencias e Engenharia do Ambiente, 
      Faculdade de Ciencias e Tecnologia da Universidade Nova de Lisboa, 2829-516, 
      Caparica, Portugal. pmcosta@fct.unl.pt
FAU - Miguel, Celia
AU  - Miguel C
FAU - Caeiro, Sandra
AU  - Caeiro S
FAU - Lobo, Jorge
AU  - Lobo J
FAU - Martins, Marta
AU  - Martins M
FAU - Ferreira, Ana M
AU  - Ferreira AM
FAU - Caetano, Miguel
AU  - Caetano M
FAU - Vale, Carlos
AU  - Vale C
FAU - DelValls, T A
AU  - DelValls TA
FAU - Costa, Maria H
AU  - Costa MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110610
PL  - United States
TA  - Ecotoxicology
JT  - Ecotoxicology (London, England)
JID - 9885956
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (Xenobiotics)
RN  - 9038-94-2 (Metallothionein)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Biological Assay
MH  - Caspase 3/genetics
MH  - Catalase/genetics
MH  - Cytochrome P-450 CYP1A1/genetics
MH  - Ecotoxicology/methods
MH  - Flatfishes/*genetics
MH  - Gene Expression Profiling
MH  - Geologic Sediments/*chemistry
MH  - Glutathione Peroxidase/genetics
MH  - HSP90 Heat-Shock Proteins/genetics
MH  - Hepatocytes/drug effects/pathology
MH  - Metallothionein/genetics
MH  - Portugal
MH  - Water Pollutants, Chemical/*toxicity
MH  - Xenobiotics/toxicity
EDAT- 2011/06/11 06:00
MHDA- 2012/03/17 06:00
CRDT- 2011/06/11 06:00
PHST- 2011/05/29 00:00 [accepted]
PHST- 2011/06/11 06:00 [entrez]
PHST- 2011/06/11 06:00 [pubmed]
PHST- 2012/03/17 06:00 [medline]
AID - 10.1007/s10646-011-0708-z [doi]
PST - ppublish
SO  - Ecotoxicology. 2011 Nov;20(8):1749-64. doi: 10.1007/s10646-011-0708-z. Epub 2011 
      Jun 10.