PMID- 21663477
OWN - NLM
STAT- MEDLINE
DCOM- 20120301
LR  - 20131121
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 14
IP  - 11
DP  - 2011 Nov
TI  - Antioxidant, antinociceptive, and anti-inflammatory properties of the ethanolic 
      extract of Combretum duarteanum in rodents.
PG  - 1389-96
LID - 10.1089/jmf.2010.0212 [doi]
AB  - The antioxidant, antinociceptive, and anti-inflammatory activities of the 
      ethanolic extract from leaves of Combretum duarteanum (EEC) were assessed in 
      rodents through in vitro tests. The antioxidant activity was investigated by 
      using thiobarbituric acid reactive species (TBARS), hydroxyl radical-scavenging, 
      and scavenging activity of nitric oxide assays. The antinociceptive activity was 
      investigated by using acetic acid-induced writhing, formalin, and hot-plate tests 
      in mice. The anti-inflammatory activity was assessed in rats by using the 
      carrageenan-induced hind-paw edema test and arachidonic acid-induced paw edema 
      test. EEC possesses a strong antioxidant potential according to the TBARS, nitric 
      oxide, and hydroxyl radical-scavenging assays; it also presented scavenger 
      activity in all in vitro tests. After intraperitoneal injection, EEC (100, 200, 
      and 400 mg/kg) significantly reduced the number of writhes (38.1%, 90.6%, and 
      97.8%, respectively) in a writhing test and the number of paw licks during phase 
      1 (30.5% and 69.5%, higher doses) and phase 2 (38.1%, 90.6%, and 97.8%, all 
      doses) of a formalin test when compared with the control group. Naloxone (1.5 
      mg/kg, intraperitoneally) antagonized the antinociceptive action of EEC (400 
      mg/kg), and this finding suggests participation of the opioid system. 
      Administration of 200 and 400 mg/kg (intraperitoneally) of EEC exhibited an 
      anti-inflammatory activity in the carrageenin test, which was based on 
      interference with prostaglandin synthesis. This finding was confirmed by the 
      arachidonic acid test. Together, these results indicate that properties of EEC 
      might be further explored in the search for newer tools to treat painful 
      inflammatory conditions, including those related to pro-oxidant states.
FAU - Gouveia, Marcos G S
AU  - Gouveia MG
AD  - Department of Physiology, Universidade Federal de Sergipe, Sao Cristovao, 
      Sergipe, Brazil.
FAU - Xavier, Maria A
AU  - Xavier MA
FAU - Barreto, Andre S
AU  - Barreto AS
FAU - Gelain, Daniel P
AU  - Gelain DP
FAU - Santos, Joao P A
AU  - Santos JP
FAU - Araujo, Adriano A S
AU  - Araujo AA
FAU - Silva, Francilene A
AU  - Silva FA
FAU - Quintans, Jullyana S
AU  - Quintans JS
FAU - Agra, Maria F
AU  - Agra MF
FAU - Cabral, Analucia G S
AU  - Cabral AG
FAU - Tavares, Josean F
AU  - Tavares JF
FAU - Silva, Marcelo S
AU  - Silva MS
FAU - Quintans-Junior, Lucindo J
AU  - Quintans-Junior LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110611
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Analgesics)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 36B82AMQ7N (Naloxone)
RN  - 3K9958V90M (Ethanol)
RN  - 9000-07-1 (Carrageenan)
SB  - IM
MH  - Analgesics/antagonists & inhibitors/*pharmacology
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Arachidonic Acid/toxicity
MH  - Carrageenan/toxicity
MH  - Combretum/*chemistry
MH  - Dose-Response Relationship, Drug
MH  - Edema/chemically induced/drug therapy
MH  - Ethanol
MH  - Hydroxyl Radical/analysis
MH  - Male
MH  - Mice
MH  - Naloxone/metabolism
MH  - Nitric Oxide/analysis
MH  - Pain/drug therapy
MH  - Pain Measurement
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Rodentia
MH  - Thiobarbituric Acid Reactive Substances/analysis
EDAT- 2011/06/15 06:00
MHDA- 2012/03/02 06:00
CRDT- 2011/06/14 06:00
PHST- 2011/06/14 06:00 [entrez]
PHST- 2011/06/15 06:00 [pubmed]
PHST- 2012/03/02 06:00 [medline]
AID - 10.1089/jmf.2010.0212 [doi]
PST - ppublish
SO  - J Med Food. 2011 Nov;14(11):1389-96. doi: 10.1089/jmf.2010.0212. Epub 2011 Jun 
      11.